Abstract
Non-human primate induced pluripotent cells (iPS cells) are useful for preclinical studies of iPS cell-based therapies and the research of primate developments. Since the initial report of iPS cells in 2006, various iPS cell induction methods have been reported. Here, we describe an efficient method for inducing iPS cells using a combination of RNA transfection and chemical compounds without using transgenes. Many kinds of marmoset cells, including difficult-to-reprogram cells, can be converted into iPS cells using this combinatorial method. Furthermore, this method can be applied to other primates, including humans.
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References
Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126(4):663–676. https://doi.org/10.1016/j.cell.2006.07.024
Hayes M, Zavazava N (2013) Strategies to generate induced pluripotent stem cells. Methods Mol Biol 1029:77–92. https://doi.org/10.1007/978-1-62703-478-4_6
Schlaeger TM, Daheron L, Brickler TR, Entwisle S, Chan K, Cianci A, DeVine A, Ettenger A, Fitzgerald K, Godfrey M, Gupta D, McPherson J, Malwadkar P, Gupta M, Bell B, Doi A, Jung N, Li X, Lynes MS, Brookes E, Cherry AB, Demirbas D, Tsankov AM, Zon LI, Rubin LL, Feinberg AP, Meissner A, Cowan CA, Daley GQ (2015) A comparison of non-integrating reprogramming methods. Nat Biotechnol 33(1):58–63. https://doi.org/10.1038/nbt.3070
Fox JG (2019) The common marmoset in captivity and biomedical research. American college of laboratory animal medicine series. Academic Press, an imprint of Elsevier, London, United Kingdom, San Diego, CA
Watanabe T, Yamazaki S, Yoneda N, Shinohara H, Tomioka I, Higuchi Y, Yagoto M, Ema M, Suemizu H, Kawai K, Sasaki E (2019) Highly efficient induction of primate iPS cells by combining RNA transfection and chemical compounds. Genes Cells 24(7):473–484. https://doi.org/10.1111/gtc.12702
Poleganov MA, Eminli S, Beissert T, Herz S, Moon JI, Goldmann J, Beyer A, Heck R, Burkhart I, Barea Roldan D, Tureci O, Yi K, Hamilton B, Sahin U (2015) Efficient reprogramming of human fibroblasts and blood-derived endothelial progenitor cells using nonmodified RNA for reprogramming and immune evasion. Hum Gene Ther 26(11):751–766. https://doi.org/10.1089/hum.2015.045
Tomioka I, Maeda T, Shimada H, Kawai K, Okada Y, Igarashi H, Oiwa R, Iwasaki T, Aoki M, Kimura T, Shiozawa S, Shinohara H, Suemizu H, Sasaki E, Okano H (2010) Generating induced pluripotent stem cells from common marmoset (Callithrix jacchus) fetal liver cells using defined factors, including Lin28. Genes Cells 15(9):959–969. https://doi.org/10.1111/j.1365-2443.2010.01437.x
Jozefczuk J, Drews K, Adjaye J (2012) Preparation of mouse embryonic fibroblast cells suitable for culturing human embryonic and induced pluripotent stem cells. J Vis Exp 64:3854. https://doi.org/10.3791/3854
Miyazaki T, Isobe T, Nakatsuji N, Suemori H (2017) Efficient adhesion culture of human pluripotent stem cells using laminin fragments in an uncoated manner. Sci Rep 7:41165. https://doi.org/10.1038/srep41165
Acknowledgments
This work was supported by the Grants in Aid from the Ministry of Education, Culture, Sports, Science and Technology of JapanMEXT/JSPS KAKENHI Grant Number 15H02360 to E.S. We would like to thank Shun Yamazaki for images of iPS cell induction.
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Watanabe, T., Sasaki, E. (2021). Efficient Induction of Primate iPS Cells Using a Combination of RNA Transfection and Chemical Compounds. In: Nagy, A., Turksen, K. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 2454. Humana, New York, NY. https://doi.org/10.1007/7651_2021_373
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DOI: https://doi.org/10.1007/7651_2021_373
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