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A Method for Encapsulation and Transplantation into Diabetic Mice of Human Induced Pluripotent Stem Cells (hiPSC)-Derived Pancreatic Progenitors

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Induced Pluripotent Stem (iPS) Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2454))

Abstract

Pancreatic islet endocrine cells generated from patient-derived induced pluripotent stem cells represent a great strategy for both disease modeling and regenerative medicine. Nevertheless, these cells inherently miss the effects of the intricate network of systemic signals characterizing the living organisms. Xenotransplantation of in vitro differentiating cells into murine hosts substantially compensates for this drawback.

Here we describe our transplantation strategy of encapsulated differentiating pancreatic progenitors into diabetic immunosuppressed (NSG) overtly diabetic mice generated by the total ablation of insulin-producing cells following diphtheria toxin administration. We will detail the differentiation protocol employed, the alginate encapsulation procedure, and the xenotransplantation steps required for a successful and reproducible experiment.

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Acknowledgments

The authors thank Prof. Berit L. Strand for expert advice and support on establishing the encapsulation method. The authors also acknowledge research funding received from the Research Council of Norway (NFR 247577 and 251041), the Novo Nordisk Foundation (NNF15OC0015054), and the Norwegian Diabetesforbundet forskningfond.

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Authors and Affiliations

  1. Department of Clinical Science, Faculty of Medicine, Center for Diabetes Research, University of Bergen, Bergen, Norway

    Luiza Ghila, Thomas Aga Legøy & Simona Chera

Authors
  1. Luiza Ghila
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  2. Thomas Aga Legøy
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  3. Simona Chera
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Corresponding author

Correspondence to Simona Chera .

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Editors and Affiliations

  1. Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada

    Andras Nagy

  2. Ottawa, ON, Canada

    Kursad Turksen

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Ghila, L., Legøy, T.A., Chera, S. (2021). A Method for Encapsulation and Transplantation into Diabetic Mice of Human Induced Pluripotent Stem Cells (hiPSC)-Derived Pancreatic Progenitors. In: Nagy, A., Turksen, K. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 2454. Humana, New York, NY. https://doi.org/10.1007/7651_2021_356

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  • DOI: https://doi.org/10.1007/7651_2021_356

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2118-9

  • Online ISBN: 978-1-0716-2119-6

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