Abstract
The generation of cardiomyocytes (CMs) from human pluripotent stem cells (hPSCs) represents a valuable tool for a myriad of in vitro applications, including drug screening, disease modeling and regenerative medicine. However, the success of these applications is dependent on the establishment of reliable, efficient, simple, and cost-effective differentiation methods. In this chapter, we describe an efficient and robust 3D platform for the generation of hPSC-CMs based on the use of a microwell culture system, which can be applied in any laboratory environment. Additionally, we will also describe protocols for the structural and functional characterization of the obtained CMs for further quality control upon differentiation.
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Acknowledgments
The authors acknowledge funding from Fundação para a Ciência e a Tecnologia (FCT), Portugal (UIDB/04565/2020) through Programa Operacional Regional de Lisboa 2020 (Project N. 007317) and the project PTDC/EMD-TLM/29728/2017. Mariana A. Branco and João P. Cotovio thank FCT for financial support (PD/BD/128376/2017 and PD/BD135500/2018, respectively).
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Branco, M.A. et al. (2020). 3D Microwell Platform for Cardiomyocyte Differentiation of Human Pluripotent Stem Cells. In: Nagy, A., Turksen, K. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 2454. Humana, New York, NY. https://doi.org/10.1007/7651_2020_336
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DOI: https://doi.org/10.1007/7651_2020_336
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