Abstract
Cleanup technology and mass spectrometric determination of sphingosine-1-phosphate (S1P) using a phosphate capture molecule are shown. The protocol is rapid, requires neither thin-layer chromatography nor liquid chromatography, and is applicable to both blood and solid tissue samples. The mass spectrometric method is also applicable to ceramide-1-phosphate.
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Acknowledgments
We thank Professor Yoh Takuwa for kind donation of SphK-overexpressed cells. This work was supported by the Strategic Support Project of Research Infrastructure Formation for Private Universities from the MEXT, Japan.
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Morishige, Ji., Yamashita, R., Tanaka, T., Satouchi, K. (2017). A Cleanup Method for Mass Spectrometric Analysis of Sphingosine- and Ceramide-1-Phosphate in Blood and Solid Tissue Using a Phosphate Capture Molecule. In: Pébay, A., Turksen, K. (eds) Sphingosine-1-Phosphate. Methods in Molecular Biology, vol 1697. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_6
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DOI: https://doi.org/10.1007/7651_2017_6
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Publisher Name: Humana Press, New York, NY
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