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Fluorescent Probes for Diagnostics of β-Galactosidase: From Micro to Macro

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Fluorescent Imaging in Medicinal Chemistry

Part of the book series: Topics in Medicinal Chemistry ((TMC,volume 34))

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Abstract

β-Galactosidase (β-gal) is an enzyme commonly served as a reporter for the examination of transcription and transfection efficiencies. Due to its overexpression in primary and metastatic ovarian cancers, β-gal was also usually regarded as a molecular target for visualizing peritoneal metastases from ovarian cancers. Moreover, β-gal has been studied as a potential therapeutic target for lactose intolerance via gene replacement therapy in recent years. Interestingly, there were some reports that β-gal has been abnormally accumulated in senescent cells, which allowing this senescence-associated β-gal to be a significant biomarker for senescence. The great significance of β-gal has attracted many researchers’ attentions in developing highly selective and sensitive approaches to monitor the activity of this enzyme in vitro and in vivo. In this review, we reported the recent development of the various materials for β-gal detection and their application in disease progression monitoring, with a focus on fluorescent probe, nanomaterials, and biomolecules. Finally, the trends for the further development of the probe for fluorescence-guided diagnosis in clinical cases and its preclinical potential value were proposed.

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Acknowledgments

We are grateful for the financial supports from the National Natural Science Foundation of China (81971678, 81671756), Key Research Project of Science and Technology Foundation of Hunan Province (2017SK2093 and 2019SK2211), Key Research Project of Science and Technology Foundation of Changsha (kq1801063), and Fundamental Research Funds for the Central Universities of Central South University (2018zzts041, 2018dcyj067).

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Authors and Affiliations

  1. Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, China

    Anyao Bi, Xinchen Jiang, Tang Gao, Shuqi Yang, Yi Liu, Xiaohui Liu & Wenbin Zeng

  2. Molecular Imaging Research Center, Central South University, Changsha, China

    Anyao Bi, Xinchen Jiang, Tang Gao, Shuqi Yang, Yi Liu, Xiaohui Liu & Wenbin Zeng

Authors
  1. Anyao Bi
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  2. Xinchen Jiang
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  6. Xiaohui Liu
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  7. Wenbin Zeng
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Editors and Affiliations

  1. Stanford University, Stanford, CA, USA

    Zhen Cheng

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Bi, A. et al. (2019). Fluorescent Probes for Diagnostics of β-Galactosidase: From Micro to Macro. In: Cheng, Z. (eds) Fluorescent Imaging in Medicinal Chemistry . Topics in Medicinal Chemistry, vol 34. Springer, Cham. https://doi.org/10.1007/7355_2019_87

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