Abstract
Standards are important for calibration procedures in fluorescence imaging and overall for enabling accurate quantification. However, due to the strong nonlinear dependence of the fluorescence signal on tissue scattering, tissue absorption and activity depth, the construction of standards becomes challenging. So far, most fluorescent standards for diffusive imaging have been based on laboratory solutions that mix scattering, absorbing and fluorescence materials to construct substances of known and stable optical properties. Herein we review the most common characteristics of diffusive imaging and outline strategies to produce materials that can serve as standards in whole body imaging applications.
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© 2008 Springer-Verlag Berlin Heidelberg
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Ntziachristos, V., Hyde, D. (2008). In-vivo Fluorescence Imaging: Applications, Future Trends & Approaches to Standardization. In: Resch-Genger, U. (eds) Standardization and Quality Assurance in Fluorescence Measurements II. Springer Series on Fluorescence, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2008_052
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DOI: https://doi.org/10.1007/4243_2008_052
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