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Screening and Determination of Gene Function Using Randomized Ribozyme and siRNA Libraries

  • Chapter
RNA Towards Medicine

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 173))

Abstract

Rapid progress in the sequencing of the genomes of model organisms, such as the mouse, rat, nematode, fly, and Arabidopsis, as well as the human genome, has provided abundant sequence information, but functions of long stretches of these genomes remain to be determined. RNA-based technologies hold promise as tools that allow us to identify the specific functions of portions of these genomes. In particular, catalytic RNAs, known also as ribozymes, can be engineered for optimization of their activities in the intracellular environment. The introduction of a library of active ribozymes into cells, with subsequent screening for phenotypic changes, can be used for the rapid identification of a gene function. Ribozyme technology complements another RNA-based tool for the determination of gene function, which is based on libraries of small interfering RNAs (siRNAs).

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Authors and Affiliations

  1. Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, 113-8656, Tokyo, Japan

    S. Matsumoto, H. Akashi & K. Taira

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  1. S. Matsumoto
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  2. H. Akashi
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  3. K. Taira
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Editors and Affiliations

  1. Institute of Chemistry/Biochemistry, Free University Berlin, Thielallee 63, 14195, Berlin, Germany

    Volker Erdmann

  2. Institute of Bioorganic Chemistry, Polish Academy of Scienes, Noskowskiego 12/14, 61-704, Poznan, Poland

    Jan Barciszewski

  3. Institute of Experimental Pathology, Molecular Neurobiology (ZMBE), University of Münster, Von-Esmarch-Str. 56, 48149, Münster, Germany

    Jürgen Brosius

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Matsumoto, S., Akashi, H., Taira, K. (2006). Screening and Determination of Gene Function Using Randomized Ribozyme and siRNA Libraries. In: Erdmann, V., Barciszewski, J., Brosius, J. (eds) RNA Towards Medicine. Handbook of Experimental Pharmacology, vol 173. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-27262-3_10

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