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Interfacing Cell Surface Receptors to Hybrid Nanopatterned Surfaces: A Molecular Approach for Dissecting the Adhesion Machinery

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Bioactive Surfaces

Part of the book series: Advances in Polymer Science ((POLYMER,volume 240))

Abstract

Over the last 20 years, integrins have proven to be key players in connecting the internal cell machinery to the extracellular environment. Because the properties of the extracellular milieu strongly influence developmental programs triggered by integrin-mediated adhesion, the development of culture platforms with tunable chemical and physical properties is important for further understanding of the complexity of integrin functions. This chapter introduces biochemically modified gold surface models that have been designed to investigate cell adhesion. Specific emphasis is placed on micellar nanolithography, a technique enabling the preparation of gold nanopatterns. Such substrates are used as an analytical tool to control the activation of single-cell surface receptors for the study of integrin-mediated adhesion and signalling in a quantitative manner.

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Acknowledgements

The author thanks Professors Joachim Pius Spatz and Reinhard Fässler for offering the opportunity and support to work in this pluridisciplinary environment. The author gratefully acknowledges Professor Michael Sixt, Doctor Sara Wickström and Doctor Herbert Schiller for helpful and interesting discussions during the preparation of the manuscript, Josephine Gibson for revising the language of the manuscript, and Max Iglesias for his help with the artwork. Doctor Julien Polleux is recipient of a Marie Curie fellowship of the European Community 7th Framework Programme.

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Authors and Affiliations

  1. Department of New Materials and Biosystems, Max Planck Institute of Metals Research, Heisenbergstraße 3, 70569, Stuttgart, Germany

    Julien Polleux

  2. Department of Molecular Medicine, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany

    Julien Polleux

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  1. Julien Polleux
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Correspondence to Julien Polleux .

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Editors and Affiliations

  1. , Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, Berlin, 12489, Germany

    Hans G. Börner

  2. UPR22-CNRS 23, Precision Macromolecular Chemistry Group, Institut Charles Sadron, rue du Loess BP 84047 69, Strasbourg, 67034, France

    Jean-Francois Lutz

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Polleux, J. (2010). Interfacing Cell Surface Receptors to Hybrid Nanopatterned Surfaces: A Molecular Approach for Dissecting the Adhesion Machinery. In: Börner, H., Lutz, JF. (eds) Bioactive Surfaces. Advances in Polymer Science, vol 240. Springer, Berlin, Heidelberg. https://doi.org/10.1007/12_2010_78

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