Abstract
The non-covalent affinity of a perfluoro chain towards similar has been exploited by many to separate fluorous tagged compounds from non-fluorous compounds by F-SPE or F-LLE. This purification strategy found its application across diverse fields including peptide and oligonucleotide synthesis where even slight inefficient couplings result in deletion sequences that are often difficult to remove from the target sequence. Two commonly employed strategies to address this problem involve end-tagging the target sequence or capping the deletion sequences with fluorous tags. Solution phase syntheses using soluble fluorous supports are easier and quicker. These approaches are reviewed here in detail.
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Acknowledgments
I would like to express my gratitude to Prof. Dennis Curran for all the support and guidance during the course of my journey through fluorous chemistry and preparation of this manuscript. I would also like to thank Fluorous Technologies Inc. especially Dr. Marvin S. Yu for introducing me to this fascinating field and for his valuable inputs during the preparation of this chapter.
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Miriyala, B. (2011). Fluorous Methods for the Synthesis of Peptides and Oligonucleotides. In: Horváth, I. (eds) Fluorous Chemistry. Topics in Current Chemistry, vol 308. Springer, Berlin, Heidelberg. https://doi.org/10.1007/128_2011_247
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DOI: https://doi.org/10.1007/128_2011_247
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