Abstract
Two friabilin components, puroindoline a and GSP-1 were expressed in Escherichia coli. Starch binding properties of the recombinant polypeptides and of friabilin extracted from wheat flour were compared in vitro. The produced proteins as well as native wheat friabilin bound to starch granules prepared from different (soft, hard and durum) wheat cultivars. Starch granules also bound specifically several wheat endosperm proteins other than friabilin
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References
Baldwin PM (2001) Starch granule-associated proteins and polypeptides: a review. Starch/Stärke 53: 475–503
Barlow KK, Butrose MS, Simmonds DH, Vesk M (1973) The nature of the starch-protein interface in wheat endosperm. Cereal Chem 50:443–454
Bloch HA, Darlington HF, Shewry PR (2001) In vitro binding of puroindolines to wheat starch granules. Cereal Chem 78:74–78
Blochet JE, Chevalier C, Forest E, Pebay-Peyroula E, Gautier MF, Joudrier P, Pezolot M, Marion D (1993) Complete amino acid sequence of puroindoline, a new basic and cystine-rich protein with a unique tryptophan-rich domain, isolated from wheat endosperm by Triton X-114 phase partitioning. FEBS Lett 329:336–340
Douliez JP, Michon T, Elmorjani K, Marion D (2000) Structure, biological and technological functions of lipid transfer proteins and indolines, the major lipid binding proteins from cereal kernels. J Cereal Sci 32:1–20
Dubriel L, Compoint JP, Marion D (1997) The interaction of puroindolines with wheat flour polar lipids determines their foaming properties. J Agr Food Chem 45:108–116
Greenblatt GA, Bettge AD, Morris CF (1995) Relationship between endosperm texture and the occurrence of friabilin and bound polar lipids on wheat starch. Cereal Chem 72:172–176
Issaly N, Solsona O, Joudrier P, Gautier MF, Moulin G, Boze H (2001). Optimization of the wheat puroindoline production in Pichia pastoris. J Appl Microbiol 90:397–406
Morris CF (2002) Puroindolines: the molecular genetic basis of wheat grain hardness. Plant Mol Biol 48:633–647
Schägger H, von Jagow G (1987) Tricine-sodium dodecyl sulphate poly-acrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166: 368–379
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Bakó, A., Gárdonyi, M., Tamás, L. (2007). In Vitro Starch Binding Experiments: Study of the Proteins Related to Grain Hardness of Wheat. In: Buck, H.T., Nisi, J.E., Salomón, N. (eds) Wheat Production in Stressed Environments. Developments in Plant Breeding, vol 12. Springer, Dordrecht. https://doi.org/10.1007/1-4020-5497-1_83
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DOI: https://doi.org/10.1007/1-4020-5497-1_83
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5496-9
Online ISBN: 978-1-4020-5497-6
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