Abstract
A set of Argentinean and Chilean cultivars of durum wheat were subjected to the following fertiliser regimes in designed field trials grown over three years: N0S0 (no applied nitrogen or sulphur fertiliser), N1S0 (applied nitrogen fertiliser only), N1S1 (both applied nitrogen and sulphur fertiliser) and N0S1 (applied sulphur fertiliser only), where N was applied as urea and S as potassium sulphate. Two protein extraction protocols were applied to each flour sample obtained from the trial, one for reduced glutenin subunits and one for the gliadins. The resultant extracts were separated by SDS-PAGE, the gels were scanned and the images analysed densitometrically. The data obtained were analysed statistically in order to determine whether the following quantities varied over cultivars and over fertiliser treatments: (i) the relative proportion of total gliadins to total glutenins (Gli:Glu ratio); (ii) the relative proportion of individual ω-gliadin proteins to other gliadin proteins; and (iii) the relative proportion of HMW to LMW subunits of glutenin (HMW:LMW ratio). Such variation would be expected in the light of previous studies in wheat and given that sequence data have shown that these components vary in molecular S-content. It was found that (a) one of the cultivars (cv. Bonerense INTA Cumenay) showed relatively high Gli:Glu ratios compared to the other cultivars; (b) the fertiliser treatment N0S0 gave higher Gli:Glu ratios than the remaining treatments in the two years where significant differences were observed; (c) two of the cultivars (cv. B. I. Cumenay and cv. Chagual INIA) generally gave higher HMW:LMW ratios than the remaining two (cv. Bonaerense INTA Facón and cv. Buck Topacio); (d) the treatments involving N-application (N1S0 and N1S1) generally showed higher HMW:LMW ratios than those without N-application (N0S0 and N0S1); (e) although effects of S-application were observed, these were inconsistent over years; (f) Cv. B. I. Cumenay showed relative stability over fertiliser treatments for the quantities evaluated, coherent with its previously observed stability for industrial quality characteristics. The findings hopefully contribute to our understanding of why cultivars differentially respond to fertiliser treatments for quality characteristics
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Brites C, Carrillo JM (2001) Influence of high molecular weight (HMW) and low molecular weight (LMW) glutenin subunits controlled by Glu-1 and Glu-3 loci on durum wheat quality. Cereal Chem 78:59–63
Fares C, Novembre G, Di Fonzo N, Galterio G, Pogna NE (1997) Relationship between storage protein composition and gluten quality in breeding lines of durum wheat (Triticum turgidum spp. durum). Agricoltura Mediterranea 127:363–368
Galterio G, Grita L, Brunori, A (1993) Pasta-making quality in Triticum durum. New indices from the ratio among protein components separated by SDS-PAGE. Plant Breed 116:541–545
Lerner SE, Cogliatti M, Ponzio NR, Seghezzo ML, Molfese ER, Rogers WJ (2004) Genetic variation for grain protein components and industrial quality of durum wheat cultivars sown in Argentina. J Cereal Sci 40:161–166
Lerner SE, Seghezzo ML, Molfese ER, Ponzio NR, Cogliatti M, Rogers WJ (2006) N- and S-fertiliser effects on grain composition, industrial quality and end-use in durum wheat. J Cereal Sci 44:2–11
MacRitchie F, Gupta RB (1993) Functionality-composition relationships of wheat flour as a result of variation in sulfur availability. Aust J Agr Res 44:1767–1774
Martinez MC, Ruiz M, Carrillo JM (2004) New B low Mr glutenin subunit alleles at the Glu-A3, Glu-B2 and Glu-B3 loci and their relationship with gluten strength in durum wheat. J Cereal Sci 40:101–107
Martinez MC, Ruiz M, Carrillo JM (2005) Effects of different prolamin alleles on durum wheat quality properties. J Cereal Sci 41:123–131
Moss HJ, Wrigley CW, MacRitchie F, Randall PJ (1981) Sulfur and Nitrogen fertilizer effects on wheat. II. Influence on grain quality. Aust J Agr Res 32:213–226
Payne PI, Holt LM, Jackson EA, Law CN (1984) Wheat storage proteins: their genetics and their potential for manipulation by plant breeding. Phil Trans R Soc B, 304:359–371
Pogna NE, Autran JC, Mellini F, Lafiandra D (1990) Chromosome 1B- encoded gliadins and glutenin subunits in durum wheat: genetics and relationship to gluten strength. J Cereal Sci 11:15–34
Rhazi L, Cazalis T, Aussenac T (2003) Sulfhidryl-disulphide changes in storage proteins of developing wheat grains: influence on the SDS-unextractable glutenin polymer formation. J Cereal Sci 38:3–13
Ruiz M, Carrillo JM (1995) Relationships between different prolamin proteins and some quality parameters in durum wheat. Plant Breed 114:40–44
San Martín N, Navarro C, Echeverría H (1990) Sulfur concentration in wheat grains in the south of the Buenos Aires Province. Boletín Técnico INTA Nˆ 99:3–7
Shewry PR, Tatham AS (1997) Mini review: disulphide bonds in wheat gluten proteins. J Cereal Sci 25:207–227
Singh NK, Donovan GR, MacRitchie F (1990) Use of sonication and size-exclusion high-performance liquid chromatography in the study of wheat flour proteins. II. Relative quantity of glutenin as a measure of breadmaking quality. Cereal Chem 67:161–170
Singh NK, Shepherd KW, Cornish GB (1991) A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. J Cereal Sci 14:203–208
Timms MF, Bottomley RC, Ellis JRS, Schofield JD (1981) The baking quality and protein characteristics of a winter wheat grown at different levels of nitrogen fertilisation. J Sci Food Agr 32:648–698
Vazquez JF, Ruiz M, Nieto-Taladriz MT, Albuquerque MM (1996) Effects on gluten strength of low Mr glutenin subunits coded by alleles at the Glu-A3 and Glu-B3 loci in durum wheat. J Cereal Sci 24:125–130
Wrigley CW, du Cros DL, Archer MJ, Downie PG, Roxburg CM (1980) The sulfur content of wheat endosperm proteins and its relevance to grain quality. Aust J Plant Physiol 7:755–766
Zadocks JC, Chang TT, Konzak CF (1979) A decimal code for the growth stages of cereals, with illustrations. Ann Appl Biol 93:221–234
Zhao FJ, Hawkesford MJ, McGrath SP (1999) Mini review: sulphur assimilation and effects on yield and quality of wheat. J Cereal Sci 30:1–17
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer
About this paper
Cite this paper
Lerner, S.E., Cogliatti, M., Ponzio, N.R., Seghezzo, M.L., Molfese, E.R., Rogers, W.J. (2007). Nitrogen-Sulphur Fertiliser Induced Changes in Storage Protein Composition in Durum Wheat. In: Buck, H.T., Nisi, J.E., Salomón, N. (eds) Wheat Production in Stressed Environments. Developments in Plant Breeding, vol 12. Springer, Dordrecht. https://doi.org/10.1007/1-4020-5497-1_67
Download citation
DOI: https://doi.org/10.1007/1-4020-5497-1_67
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5496-9
Online ISBN: 978-1-4020-5497-6
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)