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Biotechnology in Mulberry (Morus spp.) Crop Improvement: Research Directions and Priorities

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Plant Biotechnology and Molecular Markers

Abstract

Mulberry (Morus spp.) is a crop plant of economic importance in sericulture. Mulberry improvement through conventional breeding has substantially contributed to the success of sericulture industry. However, the application of biotechnology in mulberry crop improvement holds a great promise especially in those areas where conventional research has not achieved the desired success. The biotechnological research in genome characterization with isozyme and DNA markers, micropropagation, regeneration from callus, somatic hybridization, in vitro conservation technologies like slow-growth storage and cryopreservation, genetic transformation etc., have contributed to the success in mulberry improvement. Besides discussing the progress achieved so far in mulberry biotechnology, the article also emphasizes the future priorities in this direction both in terms of supportive and strategic research.

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References

  1. R.L. Hunter, C.L. Markert, Histochemical demonstration of enzymes separated by zone electrophoresis in starch gels, Science 125 (1957) 1294–1295.

    CAS  PubMed  Google Scholar 

  2. H. Hirano, Evaluation of affinities in mulberry and its relatives by peroxidase isozyme technique, JARQ 11 (1977) 228–233.

    CAS  Google Scholar 

  3. H. Hirano, K. Naganuma, Inheritance of peroxidase isozymes in mulberry (Morus spp.), Euphytica 28 (1979) 73–79.

    Article  CAS  Google Scholar 

  4. H. Hirano, Thremmetological studies of protein variation in mulberry, Bull. Sericult. Expt. Sta. 36 (1980) 67–186.

    Google Scholar 

  5. K. Katagiri, Y. Kunitomo, R.R. Davalos, E. Iwata, Hexapolids found in mulberry strains collected in Mexico, J. Seric. Sci. Jpn. 63 (1994) 425–426.

    CAS  Google Scholar 

  6. M. Venkateswaralu, B.N. Susheelamma, N. Suryanarayana, K. Sengupta, Peroxidase isozyme studies in four mulberry species introduced from Indonesia, Ind. J. Seric. 28 (1989) 271–273.

    Google Scholar 

  7. M. Venkateswaralu, B.N. Susheelamma, M.V. Rajan, P.K. Tewari, A. Sarkar, Peroxidase isozyme studies in triploids of mulberry (Morus spp.), Ind. J. Seric. 34 (1995) 153–155.

    Google Scholar 

  8. M. Venkateswaralu, B.N. Susheelamma, N. Suryanarayana, N.K Dwivedi, K. Sengupta, Peroxidase isozyme banding patterns in aneuploids of mulberry, Sericologia 29 (1989a) 99–104.

    Google Scholar 

  9. K. Katagiri, H. Hirano, H. Hirai, H. Ichikawa, Isolation of chloroplast DNA in mulberry, J. Seric. Sci. Jpn. 53 (1984) 83–84.

    CAS  Google Scholar 

  10. H. Machii, Isolation of total mulberry DNA, J. Seric. Sci. Jpn. 58 (1989) 349–350.

    CAS  Google Scholar 

  11. L. Chengfu, Z. Youzuo, Z. Yaozhou, Studies on RAPD in mulberry, Journal of Zhejiang Agric. Univ. 22 (1996) 149–151.

    Google Scholar 

  12. F. Lichun, Y. Guang Wei, Y. MaoDe, Z. Xiaoyong, X. Zhong Huai, Study of relationship among species in Morus L. using random amplified polymorphic DNA (RAPD). Scientia Agricultura Sinica 30 (1997) 52–56.

    Google Scholar 

  13. C.F. Lou, Y.Z. Zhang, J.M. Zhou, Polymorphisms of genomic DNA in parents and their resulting hybrids in mulberry Morus. Sericologia 38 (1998) 437–445.

    Google Scholar 

  14. A. Sharma, R. Sharma, H. Machii, Assessment of genetic diversity in a Morus germplasm collections using fluorescence-based AFLP markers, Theor. Appl. Genet. 101 (2000) 1049–1055.

    Article  CAS  Google Scholar 

  15. E. Bhattacharya, S.A. Ranade, Molecular distinction amongst varieties of mulberry using RAPD and DAMD profiles, BMC Plant Biology 1 (2001) 1–8.

    Article  Google Scholar 

  16. Annual Report 1997–98, Silkworm and Mulberry Germplasm Station, Hosur.

    Google Scholar 

  17. Annual Report 1999–2000, Central Sericultural Germplasm Resources Centre, Hosur.

    Google Scholar 

  18. Annual Report 2000–2001, Central Sericultural Germplasm Resources Centre, Hosur.

    Google Scholar 

  19. Annual Report 2001–02, Central Sericulural Germplasm Resources Centre, Hosur.

    Google Scholar 

  20. Annual Report 2001–2002, Central Sericultural Research and Training Instiute, Mysore.

    Google Scholar 

  21. K. Ohyama, Tissue culture in mulberry tree, JARQ 5 (1970) 30–34.

    Google Scholar 

  22. K. Ohyama, S. Oka, Effects of absicic acid and gibberellic acid on bud break of mulberry plants. J. Seric. Sci. Jpn. 44 (1975) 321–326.

    CAS  Google Scholar 

  23. K. Ohyama, S. Oka, Regeneration of whole plants from isolated shoot tips of mulberry tree. J. Seric. Sci. Jpn 45 (1976) 115–120

    Google Scholar 

  24. S. Oka, K. Ohyama, In vitro initiation of adventitious buds and its modification by high concentration of benzyladenine in leaf tissues of mulberry (Morus alba) Can. J. Bot. 59 (1981) 68–74.

    Article  Google Scholar 

  25. H.R. Kim, K.R. Patel, T.A. Thorpe, Regeneration of mulberry plantlets through tissue culture, Bot. Gazett. 146 (1985) 335–340.

    Google Scholar 

  26. P. Narayanan, S. Chakraborty, G.S. Rao, Regneration of plantlets from the callus of stem segements of mature plants of Morus alba L. Proc. Ind. Nat. Sci. Acad. B55 (1989) 469–472.

    Google Scholar 

  27. K.K. Sharma, T.A. Thorpe, In vitro propagation of mulberry (Morus alba L.) through nodal segments, Scientia Hort. 42 (1990) 307–320.

    Article  Google Scholar 

  28. P.K. Tewary, G.S. Rao, Multiple shoot formation through shoot apex culture of mulberry, Ind. J. Forestry (1990) 13: 109–111.

    Google Scholar 

  29. S. Chattopadhay, S.K. Datta, A rapid clonal propagation of mulberry tree (Morus alba) through tissue culture, Proc. Ind. Sci. Cong. Part 3 (1990) 208–209.

    Google Scholar 

  30. S. Chattopadhay, S. Chattopadhyay, S.K. Datta, Quick in vitro production of mulberry (Morus alba) plantlets for commercial purpose. Ind. J. Exp. Biol. 28 (1990) 522–525.

    Google Scholar 

  31. M.K. Raghunath, S.P. Chakraborthy, B. Roy, S.K. Sen, Micropropagation of superior hybrids through axillary bud culture in mulberry (Morus L.). Cell and Chromosome Res. 15 (1992) 12.

    Google Scholar 

  32. K. Kathiravan, A. Shajahan, A. Ganapathi, Regneration of plantlets from hypocotyl derived callus cultures of mulberry Morus alba L. Israel J Plant Sci. 43 (1995) 259–262.

    Google Scholar 

  33. K. Hayashi, S. Oka, Formation of multiple bud bodies and plant regeneration in mulberry, Morus alba, J. Seric. Sci. Jpn. 64 (1995) 117–123.

    CAS  Google Scholar 

  34. P.K. Tewary, A. Sarkar, V. Kumar, S. Chakraborthy, Rapid in vitro multiplication of high yielding mulberry (Morus spp.) genotypes V1 and S-34, Ind. J. Seric. 34 (1995) 133–136.

    Google Scholar 

  35. B. Verma, V. Patni, U. Kant, H.C. Arya, Direct plantlet regeneration from axillary buds of Morus alba L. (grafted var. — White) J. Ind. Bot. Soc. 75 (1996) 45–47.

    Google Scholar 

  36. V.A. Bapat, M. Mhatre, P.S. Rao Propagation of Morus indica L. (mulberry) by encapsulated shoot buds, Plant Cell. Rep. 6 (1987) 393–395.

    Article  Google Scholar 

  37. S.K. Pattnaik, Y. Sahoo, P.K. Chand, Efficient plant retrieval from alginate-encapsulated vegetative buds of mature mulberry trees, Scientia. Hort. 61 (1995) 227–239.

    Article  Google Scholar 

  38. Y. Sahoo, S.K. Pattnaik, P.K. Chand, Plant regeneration from callus cultures of Morus indica L. derived from seedlings and matured plants, Scientia Hort. 69 (1997) 85–98.

    Article  CAS  Google Scholar 

  39. J. Ivanica, In vitro micropropagation of mulberry, Morus nigra L. Scientia. Hort. 32 (1987) 33–39.

    Google Scholar 

  40. U. Yadav, M. Lal, V.S. Jaiswal, Micropropagation of Morus nigra L. from shoot tip and nodal explants of matured trees, Scientia Hort. 44 (1990) 61–67.

    Article  Google Scholar 

  41. A.K. Jain, S.B. Dandin, K. Sengupta, In vitro micropropagation through axillary bud multiplication in different mulberry genotypes, Plant Cell Reps. 8 (1990) 737–740.

    CAS  Google Scholar 

  42. A.K. Jain, R.K. Datta, Shoot organogenesis and plant regeneration in mulberry (Morus bombycis Koidz.): factors influencing morphogentic potential in callus cultures, Plant Cell Tiss. Org. Cult. 29 (1992) 43–50.

    Article  CAS  Google Scholar 

  43. H. Yakua, S. Oka, Plant regeneration through meristem cultures from vegetative buds of mulberry (Morus bombycis Koidz.) stored in liquid nitrogen, Ann. Bot. 62 (1988) 79–82.

    Google Scholar 

  44. M. Hossain, S.M. Rahman, A. Zaman, O.I. Joarder, R. Islam, Micropropagation of M. laevigata Wall. from matured trees. Plant Cell Rep. 11 (1992) 522–524.

    Article  Google Scholar 

  45. S.K. Pattnaik, Y. Sahoo, P.K. Chand, Micropropagation of a fruit tree M. australis Poir. Syn. M. acidosa Griff. Plant Cell Rep. 15 (1996) 841–845.

    CAS  Google Scholar 

  46. M. Mhatre, V.A. Bapat, P.S. Rao, Regneration; of plants from the culture of leaves and axillary buds in mulberry (Morus indica L.), Plant Cell Rep. 4 (1985) 78–80.

    Article  Google Scholar 

  47. S. Oka, K. Ohyama, Studies on in vitro cultures of excised buds in mulberry tress-III. Effects of agar concentration, pH and sugar of medium on the development of shoot from winter buds, J. Seric. Sci. Jpn 47 (1978) 15–20.

    Google Scholar 

  48. S. Enmoto, Preservation of genetic resource of mulberry by means of tissue culture, JARQ 21 (1987) 205–210.

    Google Scholar 

  49. S.K. Pattnaik, P.K. Chand, Rapid clonal propagation of three mulberries, Morus cathyana, Hemsl., M. lhou Koidz. and M. serrata Roxb. through in vitro culture of apical shoot buds and nodal explants from mature trees. Plant Cell Rep. 16 (1997) 503–508.

    CAS  Google Scholar 

  50. S. Oka, K. Ohyama, Induction of callus and effects of constituents of medium on callus formation of mulberry trees. J. Seric. Sci. Jpn. 42 (1973) 317–324.

    CAS  Google Scholar 

  51. K.S. Ogurstov, K.S. Atabekova, M.M. Khalmirzaev, S.H.R. Madyarov, Culturing the meristem of young mulberry on artificial nutrient medium, Shelk 1 (1986) 3.

    Google Scholar 

  52. P.K. Tewary, B.K. Gupta, G.S. Rao, In vitro studies on the growth rate of callus of mulberry (Morus alba L.), Indian. J. Forestry 12 (1989) 34–35.

    Google Scholar 

  53. T. Ohnishi, Y. Kobayashi Separation of free cells from the hypocotyl of mulberry. J. Seric. Sci. Jpn (1991a) 117–124.

    Google Scholar 

  54. T. Ohnishi, Y. Kobayashi, Suspension culture of cells from hypocotyl of mulberry, J. Seric. Sci. Jpn. 60 (1991b) 320–323.

    CAS  Google Scholar 

  55. T. Ohnishi, F. Yasukura, J. Tan, Preservation of mulberry callus by the addition of both abscisic acid (ABA) and p-aminobenzoate (PABA) in culture medium, J. Seric. Sci. Jpn. 55 (1986) 252–255.

    CAS  Google Scholar 

  56. K. Oshigane, On the redifferentiation of shoot callus to different culture in mulberry, J. Fac. Text. Sci. Tech. Shinshu Univ. Agric. Seric 107 (1989a) 38.

    Google Scholar 

  57. K. Oshigane, D. Zhuang, Effect of in vitro colchicine treatment in the culture of isolated buds in mulberry, J. Seric Sci. Jpn. 59 (1990) 187–195.

    CAS  Google Scholar 

  58. S. Rao, M.K. Raghunath, Callus initiation and plant regeneration on mulberry (Morus alba L.) cultivars, J. Swamy. Bot. Club. 10 (1993) 17–21.

    Google Scholar 

  59. F. Yasukura, T. Ohnishi, Effects of regeneration period of mulberry cultures on their growth after their storage and after the pseudo dormancy induced by storage, J. Seric. Sci. Jpn. 59 (1990) 255–258.

    Google Scholar 

  60. H. Seki, M. Takeda, K. Trutrumi, Y. Ushiki, Studies on the callus culture of mulberry trees (1) The effect of concentration of auxin and kinetin on the callus culture of mulberry stem J. Seric. Sci. Jpn 40 (1971) 81–85.

    CAS  Google Scholar 

  61. D.D. Ghugale, D.D. Kulkarni, R. Narasimhan, Effects of auxin and gibberillic acid on growth and differentiation of Morus alba and Populus nigra tissue in vitro, Ind. J. Exp. Biol. 9 (1971) 381–384.

    CAS  Google Scholar 

  62. T. Ohnishi, Y. Kobayashi, Root formation from the suspension cells of mulberry hypocotyl. J. Seric. Sci. Jpn. 60 (1991c) 505–507.

    CAS  Google Scholar 

  63. R. Islam, A. Zaman, M. Hossain, A.C. Barmar, O.I. Joarder, A.B.M.B. Hossain, Effects of growth regulators on in vitro callogenesis in Morus laevigata wall, Bull. Sericult. Res. 3 (1992) 55–58.

    Google Scholar 

  64. K.S. Jagadischandra, N. Sathyanarayana, Regeneration of plants in mulberry (Morus indica) var. Mysore Local through leaf callus culture, J. Plant Biol. 28 (2001) 147–152.

    Google Scholar 

  65. A. Kapur, S. Bhatnagar, P. Khurana, Efficient regeneration from mature leaf explants of Indian mulberry via organogenesis, Sericologia 41 (2001) 207–214.

    Google Scholar 

  66. S. Linn, D. Ji, J. Qin, In vitro production of haploid plants from mulberry (Morus) anther culture, Scientia Sinica 30 (1987) 853–863.

    Google Scholar 

  67. M. Venkateswaralu, K. Katagiri, Induction of greenish status and callus in anther culture of mulberry, Proc. Japnese Sericulture. Association (1991) Mito, Japan, Nov. 28–30. P.11

    Google Scholar 

  68. M. Sethi, S. Bose, A. Kapur, N.S. Rangaswamy, Embryo differentiation in anther culture of mulberry, Ind. J. Expt. Biol. 30 (1992) 1146–1148.

    Google Scholar 

  69. K. Katagiri, Callus induction in cultures of mulberry pollen. J. Seric. Sci. Jpn. 58 (1989b) 267–268.

    Google Scholar 

  70. K. Katagiri, M. Venkateswarulu, Induction of callus, embryo like and organ like division in pollen cultures of Indian mulberry variety Behrampur, Proc. Japnese Sericulture Association (1991a) Mito, Japan Nov. 28–30 P.30

    Google Scholar 

  71. K. Katagiri, M. Venkateswaralu, Induction and calli and organ-like structures in isolated pollen culture of mulberry, Morus australis Poiret. J. Seric. Sci. Jpn. 62 (1993) 1–6.

    CAS  Google Scholar 

  72. K. Katagiri, M. Venkateswaralu, Effects of sugars and sugar alcohols in the division of mulberry pollen in tissue culture, J. Seric. Sci. Jpn. 60 (1991b) 514–516.

    CAS  Google Scholar 

  73. P.K. Tewary, S.P. Chakraborthy, S.S. Sinha, R.K. Datta, In vitro study on pollen culture in mulberry, Acta Botanica Indica 22 (1994) 186–190.

    Google Scholar 

  74. G. Lakshmisita, S. Ravindran, Gynogenic plants from ovary cultures of mulberry (Morus indica), in: J. Prakash, RLM Pierik (Eds) Horticulture-New Technologies and applications, pp. 225–229. Kluwer Acad. Publ., Dordrecht.

    Google Scholar 

  75. T. Dennis Thomas, A.K. Bhatnagar, M.K. Razdan, S.S. Bhojwani, A reproducible protocol for the production of gynogenic haploids of mulberry, Morus alba L., Euphytica 110 (1999) 169–173.

    Google Scholar 

  76. T. Ohnish, S. Kiyama, Increase in yield of mulberry protoplasts by treatments with chemical substance, J. Seric. Sci. Jpn. 56 (1987a) 407–410.

    Google Scholar 

  77. K. Katagiri, Difference in cell division in mulberry mesophyll protoplast culture due to species and culture conditions, J. Seric.Sci. Jpn 57 (1988) 445–446.

    Google Scholar 

  78. P.K. Tewary, G. Lakshmi Sita, Protoplast isolation, purification and cultures in mulberry (Morus spp.) Sericologia 32 (1992) 651–657.

    Google Scholar 

  79. T. Ohnishi, S. Kiyama, Effects of change in temperature, pH, Ca ion concentration in the solution used for protoplast fusion on the improvement of the fusion ability of mulberry protoplasts, J. Seric. Sci. Jpn 56 (1987b) 418–421.

    CAS  Google Scholar 

  80. K. Katagiri, Colony formation in culture of mulberry mesophyll protoplasts, J. Seric. Sci. Jpn. 58 (1989a) 267–268.

    Google Scholar 

  81. W.Z. Ming, Z.H. Xu, N. Xu, H.M. Ren, Plant regeneration from leaf mesophyll protoplasts of Morus alba, Plant Physiol. Commu. 28 (1992) 248–249.

    Google Scholar 

  82. T. Ohnishi, K. Tanabe, On the protoplast fusion of mulberry and paper mulberry by electrofusion method, J. Seric. Sci. Jpn. 58 (1989) 353–354.

    Google Scholar 

  83. P.K. Chand, Y. Sahoo, S.K. Pattnaik, Artificial seeds: a novel approach to mulberry propogation, Indian Silk Sept. (1994) 33–38.

    Google Scholar 

  84. B.M. Reed, Y. Chang, Medium and long-term storage of in vitro culture of temperate and nut crops, In: Razdan M.K. and Cocking E.C, Conservation of plant genetic resources in vitro: General aspects, Science Publishers Inc. USA, 1 (1997) Pp. 67–106.

    Google Scholar 

  85. C.P. Wilkins, H.J. Newburry, J.H. Dodds, Tissue culture conservation of fruit tree FAO/IBPGR, Plant Gen. Res. Newsletter 73/74 (1988) 9–20.

    Google Scholar 

  86. K.K. Sharma, T.A. Thorpe, In vitro propagation of mulberry, Sci. Hort. 42 (1990) 307–320.

    Google Scholar 

  87. A. Sakai, Survival of twigs of woody plants at-196°C, Nature 185 (1960) 392–394.

    Google Scholar 

  88. T. Yokoyama, S. Oka, Survival of mulberry winter buds at super low temperatures, J. Seric. Sci. Jpn. 52 (1983) 263–264.

    Google Scholar 

  89. L. Wang, X. Zhang, Z. Yu, Cryopreservation of winter mulberry buds, Acta. Agric. Boreali-Sinica, 3 (1988) 103–106.

    Google Scholar 

  90. T. Niino, A. Sakai, H. Yakawa, Cryopreservation of dried shoot tips of mulberry winter buds and subsequent plant regeneration, Cryo-lett. 13 (1992b) 51–58.

    Google Scholar 

  91. T. Niino, Cryopreservation of germplasm of mulberry (Morus spp.), in: YPS Bajaj (Ed). Biotechnology in Agriculture and Forestry 32 (1995), Springer-Verlag, Berlin pp. 102–113.

    Google Scholar 

  92. T. Niino, A. Sakai, Cryopreservation of alginate coated in vitro grown shoot tips of apple, pear and mulberry, Plant Sci. 87 (1992) 199–206.

    Article  CAS  Google Scholar 

  93. T. Niino, A. Koyama, K. Shirata, S. Ohuchi, M. Suzuki, A Sakai, Long-term storage of mulberry winter buds by cryopreservation, J. Seric. Sci. Jpn. 62 (1993) 431–434.

    Google Scholar 

  94. H. Machii, Leaf disc transformation of mulberry plants (Morus alba L.) by Agrobacterium Ti plasmid, J. Seric. Sci. Jpn. 59 (1990) 105–110.

    Google Scholar 

  95. S. Oka, P.K. Tewary, Induction of hairy roots from hypocotyls of mulberry (Morus indica L.) by Japanese wild strains of Agrobacterium rhizogenes, J. Seri. Sci. Jpn. 69 (2000) 13–19.

    CAS  Google Scholar 

  96. A.K. Jain, R.M. Vincent, C.L. Nessler, Molecular characterization of hydroxymethylglutaryl-CoA reductase gene from mulberry (Morus alba L.), Plant Molecular Biology 42 (2000) 559–569.

    Article  CAS  PubMed  Google Scholar 

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  1. Central Sericultural Research and Training Institute, Srirampura, Mysore, 570 008, India

    S.B. Dandin & V. Girish Naik

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  1. Faculty of Science, Centre for Biotechnology, Jamia Hamdard, New Delhi, India

    P.S. Srivastava  & Alka Narula  & 

  2. Department of Genetics, University of Delhi South Campus, New Delhi, India

    Sheela Srivastava

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Dandin, S., Girish Naik, V. (2004). Biotechnology in Mulberry (Morus spp.) Crop Improvement: Research Directions and Priorities. In: Srivastava, P., Narula, A., Srivastava, S. (eds) Plant Biotechnology and Molecular Markers. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3213-7_14

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