Abstract
A method has been developed for the qualitative and quantitative detection of viral particles containing a lipid shell. The method is based on the specific binding of a fluorescent dye to cholesterol of the viral lipid shell. The fixed virus samples were examined using a fluorescence microscope at 1000x magnification. The method is fast, easy and reliable. It can be used to evaluate the infectious quality of virus vector batches (infectious/ total viral particle number). Further applications are the screening of cell lines for viral contaminations, determining the viral load in cell supernatants and the virus removal in downstream purification processes.
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patent pending (Wirth M. and Beer C., Procedure for quantitative determination of viral or bacterial particles having a cholesterol-containing envelope; international PCT/EP02/11640; USA US 10/033,041; Germany DE 101 50 959.6 and 10151 898.612)
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Beer, C., Wirth, M. (2005). A New Method for the Quantitative Determination of Enveloped Viral Particles. In: Gòdia, F., Fussenegger, M. (eds) Animal Cell Technology Meets Genomics. ESACT Proceedings, vol 2. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3103-3_63
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DOI: https://doi.org/10.1007/1-4020-3103-3_63
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-2791-8
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