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Molecular Breeding of Forage and Turf pp 155–164Cite as

Genetic Diversity in the Perennial Ryegrass Fungal Endophyte Neotyphodium lolii

  • Conference paper

Part of the book series: Developments in Plant Breeding ((DIPB,volume 11))

Abstract

Expressed sequence tag (EST) data for the grass fungal endophyte species Neotyphodium lolii and N. coenophialum has been used to generate a comprehensive set of simple sequence repeat (SSR) markers for the genetic analysis of the grass-endophyte symbiosis. Initial analysis of reference isolates from the perennial ryegrass endophyte N. lolii revealed low levels of genetic variation. Twenty-two EST-SSR markers were assembled into multiplex panels for in planta detection and genetic polymorphism analysis of the endophyte. The markers were shown to be endophyte-specific and effective at low mass-ratios of endophyte to plant genomic DNA. Genetic variation has been assessed in plants obtained from a globally distributed perennial ryegrass germplasm collection, as well as from Australian farms with variable incidence of endophyte-dependent livestock toxicosis and a perennial ryegrass variety containing an endophyte associated with reduced toxic effects. The reference isolates span the majority of the observed genetic variation in this sample, suggesting that relatively little genetic variation is present within the species, and that quantitative variation for expression of endophyte characters may be more associated with host grass genotypic differences. The geographical pattern of diversity of European-derived endophytes shows some similarity with the distribution of chloroplast DNA haplotype in perennial ryegrass, as may be expected for symbionts with matrilinear inheritance. This data supports the model of a monophyletic origin for N. lolii and dispersion from the Near East associated with ancient human migrations.

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References

  • Balfourier F, Imbert C, Charmet G (2000) Evidence for phylogeographic structure in Lolium species related to the spread of agriculture in Europe. A cpDNA study. Theor. Appl. Genet. 101: 131–138.

    Article  CAS  Google Scholar 

  • Breen JP (1993) Enhanced resistance to three species of aphids (Homoptera, Aphididae) in Acremonium endophyte-infected turfgrasses. J. Econ. Entomol. 86: 1279–1286.

    Google Scholar 

  • Christensen MJ, Easton HS, Simpson WR, Tapper BA (1998) Occurrence of the fungal endophyte Neotyphodium coenophialum in leaf blades of tall fescue and implications for stock health. N.Z. J. Agr. Res. 41: 595–602.

    Google Scholar 

  • Christensen MJ, Leuchtmann A, Rowan DD, Tapper BA (1993) Taxonomy of Acremonium endophytes of tall fescue (Festuca arundinacea), meadow fescue (F. pratensis) and perennial ryegrass (Lolium perenne). Mycol. Res. 97: 1083–1092.

    Google Scholar 

  • Dean FB, Hosono S, Fang L, Wu X, Fawad Faruqi A, Bray-Ward P, Sun Z, Zong Q, Du Y, Du J, Driscoll M, Song W, Kingsmore SF, Egholm M, Lasken RS (2002) Comprehensive human genome amplification using multiple displacement amplification. Proc. Natl.Acad. Sci USA 99: 5261–5266.

    CAS  PubMed  Google Scholar 

  • Easton HS, Latch GCM, Tapper BA, Ball OJP (2002) Ryegrass host genetic control of concentrations of endophyte-derived alkaloids. Crop Sci. 42: 51–57.

    CAS  PubMed  Google Scholar 

  • Elbersen HW, West CP (1996) Growth and water relations of field-grown tall fescue as influenced by drought and endophyte. Grass Forage Sci. 51: 333–342.

    Google Scholar 

  • Gallagher RT, Hawkes AD, Steyn PS, Vleggaar R (1984) Tremorgenic neurotoxins from perennial ryegrass causing ryegrass staggers disorder of livestock: structure elucidation of lolitrem B. J. Chem. Soc. Chem. Comm. 9: 614–616.

    Google Scholar 

  • Groppe K, Boller T (1997) PCR assay based on a microsatellite-containing locus for detection and quantification of Epichloëc endophytes in grass tissue. Appl. Env. Microbiol. 63: 1543–1550.

    CAS  Google Scholar 

  • Guthridge KM, McFarlane NM, Ciavarella TA, Batley J, Jones ES, Smith KF, Forster JW (2003) Molecular marker-based analysis of morphological traits in perennial ryegrass (Lolium perenne L.). In: Abstracts of Molecular Breeding of Forage and Turf, Third International Symposium, p.58. May 18–22, Dallas, Texas and Ardmore, Oklahoma, USA.

    Google Scholar 

  • Jaccoud D, Peng K, Feinstein D, Kilian A (2001) Diversity arrays: a solid state technology for sequence information-independent genotyping. Nucl. Acids. Res. 29(4): e25.

    Article  Google Scholar 

  • Jones ES, Dupal MP, Kölliker R, Drayton MC, Forster JW (2001) Development and characterisation of simple sequence repeat (SSR) markers for perennial ryegrass (Lolium perenne L.). Theor. Appl. Genet. 102: 405–415.

    Article  CAS  Google Scholar 

  • Keogh RG, Tapper BA, Fletcher RH (1996) Distributions of the fungal endophyte Acremonium lolii, and of the alkaloids lolitrem B and peramine, within perennial ryegrass. N.Z. J. Agr. Res. 39: 121–127.

    CAS  Google Scholar 

  • Leuchtmann A (1994) Isozyme relationships of Acremonium endophytes from 12 Festuca species. Mycol. Res. 98: 25–33.

    CAS  Google Scholar 

  • Malinowski DP, Belesky DP (1999) Neotyphodium coenophialum-endophyte infection affects the ability of tall fescue to use sparingly available phosphorus. J. Plant. Nutr. 22: 835–853.

    CAS  Google Scholar 

  • Moon CD, Scott B, Schardl CL, Christensen MJ (2000) The evolutionary origins of Epichloë endophytes from annual ryegrasses. Mycologia 92: 1103–1118.

    Google Scholar 

  • Moon CD, Tapper BA, Scott B (1999) Identification of Epichloë endophytes in planta by a microsatellite-based PCR fingerprinting assay with automated analysis. Appl. Env. Microbiol. 65: 1268–1279.

    CAS  Google Scholar 

  • Prestidge RA, Gallagher RT (1988) Endophyte fungus confers resistance to ryegrass: Argentine stem weevil larval studies. Ecol. Entomol. 13: 429–435.

    Google Scholar 

  • Rowan DD, Gaynor DL (1986) Isolation of feeding deterrents against Argentine stem weevil from ryegrass infected with the endophyte Acremonium loliae. J. Chem. Ecol. 12: 647–658.

    Article  CAS  Google Scholar 

  • Schardl CL, Leuchtmann A, Tsai HF, Collett MA, Watt DM, Scott DB (1994) Origin of a fungal symbiont of perennial ryegrass by interspecific hybridization of a mutualist with the ryegrass choke pathogen, Epichloë typhina. Genetics 136: 1307–1317.

    CAS  PubMed  Google Scholar 

  • Siegel MR, Latch GCM, Bush LP, Fannin FF, Rowan DD, Tapper BA, Bacon CW, Johnson MC (1990) Fungal endophyte infected grass: alkaloid accummulation and aphid response. J. Chem. Ecol. 16: 3301–3316.

    Article  CAS  Google Scholar 

  • Tsai HF, Liu JS, Staben C, Christensen MJ, Latch GCM, Siegel MR, Schardl CL (1994) Evolutionary diversification of fungal endophytes of tall fescue grass by hybridization with Epichloë species. Proc. Natl. Acad. Sci. U.S.A. 91: 2542–2546.

    CAS  PubMed  Google Scholar 

  • van Zijll de Jong E, Guthridge KM, Spangenberg GC, Forster JW (2003a) Development and characterization of EST-derived simple sequence repeat (SSR) markers for pasture grass endophytes. Genome 46: 277–290.

    PubMed  Google Scholar 

  • van Zijll de Jong E, Guthridge KM, Batley J, Edwards D, Spangenberg GC, Forster JW (2003b) EST-SSR sequence variation in Neotyphodium grass endophytes. Genomics of plant-symbiont relations. P5. MBFT2003, Dallas, Texas

    Google Scholar 

  • van Zijll de Jong E, Bannan NR, Batley J, Spangenberg GC, Smith KF, Forster JW (2003c) Genetic diversity in the perennial ryegrass fungal endophyte Neotyphodium lolii. In: Abstracts of Molecular Breeding of Forage and Turf, Third International Symposium, p.7. May 18–22, Dallas, Texas and Ardmore, Oklahoma, USA.

    Google Scholar 

  • van Zijll de Jong E, Kilian A, Batley J, Spangenberg GC, Forster JW (2003d) Diversity array technology for co-genotyping of the perennial ryegrass-Neotyphodium lolii interaction. Genomics of plant-symbiont relations. In: Abstracts of Molecular Breeding of Forage and Turf, Third International Symposium, p.3. May 18-22, Dallas, Texas and Ardmore, Oklahoma, USA.

    Google Scholar 

  • Welty RE, Azevedo MD, Cooper TM (1987) Influence of moisture content, temperature and length of storage on seed germination and survival of endophytic fungi in seeds of tall fescue and perennial ryegrass. Phytopathology 77: 893–900.

    Google Scholar 

  • White JF, Morgan-Jones G, Morrow AC (1993) Taxonomy, life cycle, reproduction and detection of Acremonium endophytes. Agr. Ecosyst. Environ. 44: 13–37.

    Article  Google Scholar 

  • Wilkinson HH, Siegel MR, Blankenship JD, Mallory AC, Bush LP, Schardl CL (2000) Contribution of fungal loline alkaloids to protection from aphids in a grass-endophyte mutualism. Mol. Plant. Microbe. In. 13: 1027–1033.

    CAS  Google Scholar 

  • Yates SG, Plattner RD, Garner GB (1985) Detection of ergopeptine alkaloids in endophyte infected, toxic Ky-31 tall fescue by mass spectrometry/mass spectrometry. J. Agr. Food Chem. 33: 719.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Plant Biotechnology Centre,Primary Industries Research Victoria, Department of Primary Industries, La Trobe University, Bundoora, Victoria, 3086, Australia

    Eline van Zijll de Jong, Jacqueline Batley, German C. Spangenberg & John W. Forster

  2. Primary Industries Research Victoria,Department of Primary Industries, Pastoral and Veterinary Institute, Private Bag 105, Hamilton, Victoria, 3300, Australia

    Nathaniel R. Bannan & Kevin F. Smith

  3. Centre for Environmental Stress and Adaptation Research, La Trobe University, Bundoora, Victoria, 3086, Australia

    Kathryn M. Guthridge

  4. CRC for Molecular Plant Breeding, Australia

    Eline van Zijll de Jong, Nathaniel R. Bannan, Jacqueline Batley, German C. Spangenberg, Kevin F. Smith & John W. Forster

Authors
  1. Eline van Zijll de Jong
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  2. Nathaniel R. Bannan
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  3. Jacqueline Batley
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  4. Kathryn M. Guthridge
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  5. German C. Spangenberg
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  6. Kevin F. Smith
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  7. John W. Forster
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Editor information

Editors and Affiliations

  1. Forage Improvement Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma, USA

    Andrew Hopkins , Zeng-YU Wang , Rouf Mian  & Mary Sledge , ,  & 

  2. USDA-ARS, Corvallis, Oregon, USA

    Reed E. Barker

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© 2004 Kluwer Academic Publishers

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van Zijll de Jong, E. et al. (2004). Genetic Diversity in the Perennial Ryegrass Fungal Endophyte Neotyphodium lolii. In: Hopkins, A., Wang, ZY., Mian, R., Sledge, M., Barker, R.E. (eds) Molecular Breeding of Forage and Turf. Developments in Plant Breeding, vol 11. Springer, Dordrecht. https://doi.org/10.1007/1-4020-2591-2_14

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  • DOI: https://doi.org/10.1007/1-4020-2591-2_14

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-1-4020-1867-1

  • Online ISBN: 978-1-4020-2591-4

  • eBook Packages: Springer Book Archive

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