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A Paradigm Shift in Cryopreservation: Molecular-Based Advances to Improve Outcome

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Cryogenic Engineering

Part of the book series: International Cryogenics Monograph Series ((ICMS))

Abstract

First-generation strategies of cryopreservation developed in the mid 1950s focused on the maintenance of structural integrity of cells through inclusion of penetrating cryoprotectants and management of ice and chemo-osmotic perturbations. There is now an increasing body of evidence suggesting that elevated levels of cryoprotective agents impact and alter the cellular proteome, genome, and fragmentome. Accordingly, second-generation cryopreservation strategies consider a combination of first-generation principles with new concepts in preservation solution design that reduce the effects of preservation-induced oxidative stresses. This chapter provides the background for this change in strategies.

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Authors and Affiliations

  1. Bioengineering and Biology Department, The State University of New York at Binghamton, Binghamton, NY, 13902, USA

    J.M. Baust

  2. Biological Sciences and Bioengineering Department, The State University of New York at Binghamton, Binghamton, NY, 13902, USA

    J.G. Baust

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Editors and Affiliations

  1. Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO, 80309, USA

    Klaus D. Timmerhaus

  2. Cryogenic Materials, Inc., 2625 Iliff, Boulder, CO, 80305, USA

    Richard P. Reed

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Baust, J., Baust, J. (2007). A Paradigm Shift in Cryopreservation: Molecular-Based Advances to Improve Outcome. In: Timmerhaus, K.D., Reed, R.P. (eds) Cryogenic Engineering. International Cryogenics Monograph Series. Springer, New York, NY. https://doi.org/10.1007/0-387-46896-X_14

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