Abstract
Flux distribution for a wild and a mutant strain of Saccharomyces cerevisiae are compared and investigated in terms of metabolic flux calculation and thermodynamic analysis of central metabolism under anaerobic conditions. Starting from a redundant set of measured rates obtained from batch cultures on glucose or fructose as carbon source, an original data reconciliation technique associated with the calculation of metabolic flux is used. Comparative analysis of carbon split in the metabolic network for the mutant yeast strain lacking the glucose6P-dehydrogenase (CD101-1A) and for the reference wild strain (ATCC 7754) allows to conclude that the pentose phosphate is in priority devoted to its anabolic function rather than to the production of NADPH cofactors. This last function seems to be as well assumed by the specific NADP acetaldehyde dehydrogenase enzyme; this explains the significantly higher production of acetate by the mutant strain.
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Urrieta-Saltijeral, J.M., Dussap, C.G., Pons, A., Creuly, C., Gros, J.B. (2001). Metabolic Flux Modelling as a Tool to Analyse the Behavior of a Genetically Modified Strain of Saccharomyces Cerevisiae. In: Hofman, M., Thonart, P. (eds) Engineering and Manufacturing for Biotechnology. Focus on Biotechnology, vol 4. Springer, Dordrecht. https://doi.org/10.1007/0-306-46889-1_9
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DOI: https://doi.org/10.1007/0-306-46889-1_9
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