Abstract
There is a shift from necrosis to apoptosis when batch cultures of Spodoptera frugiperda are grown in the low turbulence environment of NASA’s High-Aspect Rotating-Wall Vessel (HARV) versus a shaker flask. The NASA vessel was developed for the cultivation of animal cells in an environment which simulates microgravity on earth. Fluorescence microscopy with the DNA stains acridine orange and ethidium bromide showed 33% of S. frugiperda cells were apoptotic in the HARV when the viability was 50%, while cells grown in shaker flask had no more than 11% apoptotic cells at the same viability. We believe that the shift to apoptosis in the HARV is primarily the result of reduced hydrodynamic forces.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Clejan. S., O’Connor, K.C., Cowger, N.L., Cheles. M.K.. Haque, S., and Primavera, A. (1996) Effects of simulated microgravity on DU 145 human prostate carcinoma cells. Biotechnol. Bioeng 50, 587–597
Francis, K.M., O’Connor. K.C., and Spaulding, G.F. (1997) Cultivation of fall armyworm ovary cells in simulated microgravity. In Vitro Cell Dev. Biol. 33, 332–336.
Cowger, N.I., O’Connor. K.C., and Bivins. J.E. (1997) Influence of simulated microgravity on the longevity of insect-cell culture. Enzyme Microb. Technol. 20, 326–332.
Palli, S.R., Sohi. S.S., Cook, B.J., Primavera. M., and Retnakaran. A. (1997) Screening of 12 continuous cell lines for apoptosis, in K. Maramorosch and J. Mitsuhashi (eds.), Invertebrate Cell Culture: Novel Directions and Biotechnology Applications, Science Publishers, Inc., Enfield, New Hampshire, pp. 53–60.
Duke. R.C. and Cohen. J.J. (1992) Quantitation of apoptotic index and cell viability using fluorescent dyes, in J.E. Coligan, A.M. Kruisbeek, D.H. Margulies, E.M. Shevach, and W. Strober (eds.), Current Protocols in Immunology, Greene Publishing Associates and Wiley-lnterscience, New York. pp. 3.17.1–3.17.3.
Cowger, N.L. and O’Connor, K.C. (1997) Application of simulated microgravity to insect-cell culture, in K. Maramorosch and J. Mitsuhashi (eds.), Invertebrate Cell Culture: Novel Directions and Biotechnology Applications, Science Publishers, Inc., Enfield, New Hampshire, pp. 131–138.
Al-Rubeai, M., Singh. R.P., Goldman. M.H., and Emery, A.N. (1995) Death mechanisms of animal cells in conditions of intensive agitation. Biotechnol Bioeng. 45, 463–472.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1998 Kluwer Academic Publishers
About this chapter
Cite this chapter
Cowger, N., O’Connor, K. (1998). Enhanced Apoptosis in Insect Cells Ciltivated in Simulated Microgravity. In: Merten, OW., Perrin, P., Griffiths, B. (eds) New Developments and New Applications in Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/0-306-46860-3_42
Download citation
DOI: https://doi.org/10.1007/0-306-46860-3_42
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-5016-3
Online ISBN: 978-0-306-46860-5
eBook Packages: Springer Book Archive