Abstract
There is ample evidence that ischaemia is associated with partial denervation of the detrusor muscle and that this is responsible for much of its abnormal contractile behaviour, resulting in bladder dysfunction (instability). In guineapig nerves are very susceptible to the ischaemic damage as compared to the muscle cells. The purpose of this study was to assess the neuroprotection afforded by taurine on guinea-pig detrusor under ischaemic-like conditions. Guinea-pig detrusor strips were subjected for 60 min to ischaemic-like conditions, followed by 150 min reperfusion. Intrinsic nerves underwent every 30 min electrical field stimulation (EFS) by 5-s trains of square voltage pulses of 0.05 ms duration (15 Hz, 50 V). Detrusor strips were perfused with 0.1, 1, 3 or 10 mM taurine during the ischaemia-like exposure and the first 30 min of reperfusion. Taurine (1 and 3 mM) significantly improved the response of the strips to EFS both at the end of ischaemia and reperfusion. On the contrary, neither 0.1 nor 10 mM taurine had significant effects. It is concluded that taurine can partially counteract the ischaemia-reperfusion injury in the guineapig urinary bladder.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Chan, P. H., Schmidley, J. W., Fishman, R. A., and Longar, S. M., 1984, Brain injury, edema, and vascular permeability changes induced by oxygen-derived free radicals. Neurology 34: 315–320.
Craggs, M.D., and Stephenson, J.D., 1986, A non-cholinergic urinary bladder mechanism in New World primates. J. Physiol. (London) 377: 341–345.
Dunn, P.M., and Blakeley, A.G., 1988, Suramin: a reversible P2-purinoceptor antagonist in the mouse vas deferens. Br. J. Pharmacol. 93: 243–245.
Ferrari, R., Agnoletti, L., Comini, L., Gaia, G., Bachetti, T., Cargnoni, A., Ceconi, C., Curello, S., and Visioli O., 1998, Oxidative stress during myocardial ischaemia and heart failure. Eur. Heart J. 19(B): B 2–11.
Hoyle, C.H.V., and Bumstock, G., 1993, Postganglionic efferent transmission in the bladder and urethra. In Nervous control of the urogenital system. Part of the book series: the autonomic nervous system (C.A. Maggi, ed.), Harwood Academic Publishers, London, pp. 349–381.
Kasakov, L., and Bumstock, G., 1982, The use of a slowly degradable analog, alpha, beta-methylene ATP, to produce desensitisation of the P2-purinoceptor: effect on non-adrenergic, non-cholinergic responses of the guinea-pig urinary bladder. Eur. J. Pharmacol. 86: 291–294.
Krell, R.D., Mccoy, J.L., and Ridley, P.T., 1981; Pharmacological characterization of the excitatory innervation to the guinea-pig urinary bladder in vitro: evidence for both cholinergic and non-adrenergic-non-cholinergic neurotransmission. Br. J. Pharmacol. 74: 15–22.
Leff, P., Wood, B.E., and 0'Connor, S.E., 1990, Suramin is a slowly-equilibrating but competitive antagonist at P2-receptors in the rabbit isolated ear artery. Br. J. Pharmacol. 101: 645–649.
Levin, R.M., Ruggieri, M.R., and Wein, A.J., 1986; Functional effects of the purinergic innervation of the rabbit urinary bladder. J Pharmacol. Exp. Ther. 236: 452–457.
Maggi, C.A., Meli, A., and Santicioli, P., 1987, Neuroeffector mechanisms in the voiding cycle of the guinea-pig urinary bladder. J Auton. Pharmacol. 7: 295–308.
Maxwell, S.R., and Lip, G.Y., 1997, Reperfusion injury: a review of the pathophysiology, clinical manifestations and therapeutic options. Int. J Cardiol. 58: 95–117.
Michalk, D.V., Wingenfeld, P., Licht, C., Ugur, T., and Siar, L.F., 1996, The mechanisms of taurine mediated protection against cell damage induced by hypoxia and reoxygenation. In Taurine 2. Advances in Experimental Medicine and Biology (R.J. Huxtable and D.V. Michalk, eds.), Plenum Press, New York and London, pp. 223–232.
Palmi, M., Youmbi, G., Fusi, F., Sgaragli, G.P., Dixon, H.B.F., Frosini, M., and Tipton, K.F., 1999, Potentiation of mitochondrial Ca2+ sequestration by taurine. Biochem. Pharmacol. 58: 1123–1131.
Pessina, F., McMurray, G., Wiggin, A., and Brading, A.F., 1997, The effect of anoxia and glucose-free solutions on the contractile response of guinea-pig detrusor strips to intrinsic nerve stimulation and the application of excitatory agonist. J Urol. 157: 2375–2380.
Schaffer, S.W., Ballard, C., and Azuma, L., 1994, Mechanisms underlying physiological and pharmacological actions of taurine on myocardial calcium transport. In Taurine in health and Disease. Advances in Experimental Medicine and Biology (R.J. Huxtable and D.V. Michalk., eds.), Plenum Press, New York and London, pp. 171–196.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Kluwer Academic Publishers
About this chapter
Cite this chapter
Pessina, F., Matteucci, G., Esposito, L., Gorelli, B., Valoti, M., Sgaragli, G. (2002). Protection of Intrinsc Nerves of Guinea-Pig Detrusor Strips Against Anoxia/Glucopenia and Reperfusion Injury by Taurine. In: Della Corte, L., Huxtable, R.J., Sgaragli, G., Tipton, K.F. (eds) Taurine 4. Advances in Experimental Medicine and Biology, vol 483. Springer, Boston, MA. https://doi.org/10.1007/0-306-46838-7_37
Download citation
DOI: https://doi.org/10.1007/0-306-46838-7_37
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-306-46447-8
Online ISBN: 978-0-306-46838-4
eBook Packages: Springer Book Archive