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A polysaccharide from the fruiting bodies of Agaricus blazei Murill induces caspase-dependent apoptosis in human leukemia HL-60 cells

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Tumor Biology

Abstract

Polysaccharides are the major active ingredients of fungus Agaricus blazei for treating and preventing cancer. However, there are no reports showing anti-tumor activity of A. blazei polysaccharides (ABP) on human leukemia (HL)-60 cells in vitro and in vivo. In this study, we demonstrated that ABP efficiently inhibited proliferation of cultured HL-60 cells, which was associated with the induction of apoptosis. The increase in ABP-induced apoptosis was accompanied by loss of mitochondria membrane potential (∆Ψm), cytochrome c release from the mitochondria, activation of caspase-3, degradation of poly(ADP-ribose) polymerase (PARP), and the elevated ratio of Bcl-2-associated X (Bax)/B-cell lymphoma 2 (Bcl-2). Moreover, z-DEVD-fmk, a caspase-3 inhibitor, reversed the cytotoxic effects and apoptotic characteristics induced by ABP in HL-60 cells. Furthermore, we confirmed that ABP could obviously inhibit the solid cancer growth of leukemia HL-60 in tumor xenograft model. These data demonstrated that ABP effectively induced the apoptosis of HL-60 cells via a signaling cascade of mitochondrial caspase-3-dependent pathway.

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Authors and Affiliations

  1. The Center of Tumor Research and Therapy, Shandong Provincial Hospital to Shandong University, Jinan, 250021, Shandong Province, China

    Xiaohui Li & Junqing Han

  2. Department of Radiology, Tangdu Hospital, Fourth Military Medical University, Xi’an, 710038, Shaanxi Province, China

    Xin Zhao

  3. Department of Respiratory, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, Henan Province, China

    Hongmin Wang

  4. Department of Hematology, Tangdu Hospital, Fourth Military Medical University, Xi’an, 710038, Shaanxi Province, China

    Li Liu

  5. Department of Cardiovascular Surgery, Henan Provincial People’s Hospital, Zhengzhou, 450003, Henan Province, China

    Xiaohui Li

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  1. Xiaohui Li
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  2. Xin Zhao
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  3. Hongmin Wang
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  4. Junqing Han
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  5. Li Liu
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Correspondence to Junqing Han.

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Xiaohui Li and Xin Zhao contributed equally to this article.

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Li, X., Zhao, X., Wang, H. et al. A polysaccharide from the fruiting bodies of Agaricus blazei Murill induces caspase-dependent apoptosis in human leukemia HL-60 cells. Tumor Biol. 35, 8963–8968 (2014). https://doi.org/10.1007/s13277-014-2137-0

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  • DOI: https://doi.org/10.1007/s13277-014-2137-0

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