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EphB4 regulates the growth and migration of pancreatic cancer cells

  • Research Article
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Tumor Biology

Abstract

Pancreatic cancer is a serious threat to human life. Moreover, its treatment is complicated and its prognosis is very poor. Therefore, a new method for the diagnosis and treatment of pancreatic cancer is very essential. In this study, a eukaryotic expression plasmid targeting EphB4 was constructed and transfected into PANC-1 pancreatic cancer cells to investigate the inhibition of cell growth and the progression of iRNA against EphB4. This study provides the basis for the gene therapy of pancreatic cancer. The recombinant eukaryotic EphB4 expression plasmid, pSIREN-RetroQ-ZsGreen-EphB4 and a negative control plasmid, pSIREN-RetroQ-ZsGreen-N, were constructed. At 48 h after transfection, the relative messenger RNA (mRNA) and protein levels of EphB4 were measured by RT-PCR and western blot. The proliferation of the transfected cells was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, while cell migration ability was analyzed using the scratch migration assay. At 48 h after transient transfection, EphB4 mRNA expression was significantly decreased in transfected PANC-1 cells as compared to the control group (P < 0.01). In vitro, inhibition of EphB4 expression weakened the proliferation and cell migration ability of PANC-1 cells compared to the control group. The small interfering RNA (siRNA) eukaryotic expression plasmid vector targeting EphB4 was successfully constructed and effectively transfected into PANC-1 cells. The recombinant plasmid can inhibit the expression of EphB4 mRNA and protein in PANC-1 cells, as well as cell growth and migration.

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Authors and Affiliations

  1. Department of Oncology, Second Hospital of Dalian Medical University, Dalian, China, 116023

    Man Li, Jinbo Zhao, Jinjing Qiao & Chen Song

  2. Department of Breast Surgery, Second Hospital of Dalian Medical University, Dalian, China, 116023

    Zuowei Zhao

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  1. Man Li
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  2. Jinbo Zhao
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  3. Jinjing Qiao
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  4. Chen Song
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  5. Zuowei Zhao
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Correspondence to Man Li or Zuowei Zhao.

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Li, M., Zhao, J., Qiao, J. et al. EphB4 regulates the growth and migration of pancreatic cancer cells. Tumor Biol. 35, 6855–6859 (2014). https://doi.org/10.1007/s13277-014-1937-6

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  • DOI: https://doi.org/10.1007/s13277-014-1937-6

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