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Optimization of catalase production and purification and characterization of a novel cold-adapted Cat-2 from mesophilic bacterium Serratia marcescens SYBC-01

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Abstract

Maximum catalase production by mesophilic bacterium Serratia marcescens SYBC-01 was obtained by an optimization of culture medium and conditions. A novel cold-adapted catalase from the strain was purified and characterized. The Cat-2 without peroxidase activity was a homodimer with a molecular mass of 154 kDa, consisting of two identical subunits of about 70 kDa. Its apparent Km and Vmax value were 29.7 mM and 80,925 U/mg of protein, respectively. The Cat-2 exhibited maximal activity at pH 7.0, being relatively stable in alkaline conditions. The enzyme was most active at approximately 20°C and had 73.8% activity at 0°C. After incubation at 60°C for 60 min, the enzyme still maintained 75% of its initial activity. The Cat-2 displayed relatively higher thermostability compared to that of other cold-adapted and some mesophilic catalases.

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Acknowledgments

This work was financially supported by the National High Technology and Development Program of China (863 Program, grant No., 2010AA101501).

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Lihu Road 1800, Wuxi, 214122, Jiangsu, China

    Hua-Wei Zeng, Yu-Jie Cai, Xiang-Ru Liao, Si-Liang Qian & Feng Zhang

  2. Hanbon Science & Technology Co. Ltd., Huaian, 223001, China

    Da-Bing Zhang

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  1. Hua-Wei Zeng
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  2. Yu-Jie Cai
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  3. Xiang-Ru Liao
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  4. Si-Liang Qian
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  5. Feng Zhang
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Correspondence to Yu-Jie Cai or Xiang-Ru Liao.

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Zeng, HW., Cai, YJ., Liao, XR. et al. Optimization of catalase production and purification and characterization of a novel cold-adapted Cat-2 from mesophilic bacterium Serratia marcescens SYBC-01. Ann Microbiol 60, 701–708 (2010). https://doi.org/10.1007/s13213-010-0116-2

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  • DOI: https://doi.org/10.1007/s13213-010-0116-2

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