Skip to main content
Log in

Antioxidant activities and phenolic compounds of date plum persimmon (Diospyros lotus L.) fruits

  • Original Article
  • Published:
Journal of Food Science and Technology Aims and scope Submit manuscript

Abstract

In the present study, phenolic compounds are extracted from the date plum persimmon fruits using water, methanol and acetone as solvents. Antioxidant activities of the phenolic extracts are measured using four different tests, namely, DPPH, hydroxyl radical scavenging activities, chelating and reducing power assays. All the extracts show dose dependent DPPH radical scavenging activity, reducing and chelating powers and moreover, they are well correlated with the total phenolic and total flavonoid substances, suggesting direct contribution of phenolic compounds to these activities. In further, the extracts are identified and quantified by HPLC-ECD. Results show that gallic acid is the most abundant phenolic compound, with amounts ranging between 45.49and 287.47 μg/g dry sample. Myricetin is the dominant flavonoid in all extracts. Its level varied from 2.75 μg/g dry sample in acetone extract to 5.28 μg/g dry sample in water extract. On the basis of the results obtained, the date plum persimmon fruits phenolic extract is a potential source of natural antioxidants owing to its significant antioxidant activities.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Ayaz FA, Kadioğlu A (1999) Fatty acid compositional changes in developing persimmon (Diospyros lotus L.) fruit. New Zeal J Crop Hort 27:257–261

    Article  CAS  Google Scholar 

  • Ayaz FA, Kadioğlu A, Reunanen M (1997) Changes in phenolic acid contents of Diospyros lotus L. during fruit development. J Agric Food Chem 45(7):2539–2541

    Article  CAS  Google Scholar 

  • Cao W, Chen WJ, Suo ZR, Yao YP (2008) Protective effects of ethanolic extracts of buckwheat groats on DNA damage caused by hydroxyl radicals. Food Res Int 41:924–925

    Article  CAS  Google Scholar 

  • Carter P (1971) Spectrophotometric determination of serum iron at the submicrogram level with a new reagent (ferrozine). Anal Biochem 40(2):450–458

    Article  CAS  Google Scholar 

  • Chung SK, Osawa T, Kawakishi S (1997) Hydroxyl radical-scavenging effects of species and scavengers from brown mustard (Brassica nigra). Biosci Biotechnol Biochem 61:118–123

    Article  CAS  Google Scholar 

  • Dowd LE (1959) Spectrophotometric determination of quercetin. Anal Chem 31(7):1184–1187

    Article  CAS  Google Scholar 

  • Dreher D, Junod AF (1996) Role of oxygen free radicals in cancer development. Eur J Cancer 32:30–38

    Article  Google Scholar 

  • Ebrahimzadeh MA, Pourmorad F, Bekhradnia AP (2008) Iron chelating activity, phenol and flavonoid content of some medicinal plants from Iran. Afr J Biotechnol 7(18):3188–3192

    CAS  Google Scholar 

  • Evans CR, Miller N, Paganga G (1997) Antioxidant properties of phenolic compounds. Trends Plant Sci 4:152–159

    Article  Google Scholar 

  • Gao H, Cao W, Liang Y, Cheng N, Wang BN, Zheng JB (2010) Liquid chromatographic determination of phenol and thymol in honey using electrochemical detection. Chromatographia 72:361–363

    Article  CAS  Google Scholar 

  • Glew RH, Ayaz FA, Millson M, Huang HS, Chuang LT, Sanz C, Golding JB (2005) Changes in sugars, acids and fatty acids in naturally parthenocarpic date plum persimmon (Diospyros lotus L.) fruit during maturation and ripening. Eur Food Res Tech 221:113–118

    Article  CAS  Google Scholar 

  • Gorinstein S, Zemser M, Weisz M, Halevy S, Deutsch J, Tilis K, Feintuch D, Guerra N, Fisherman M, Bartnikowska E (1994) Fluorometric analysis of phenolics in persimmons. Biosci Biotechnol Biochem 58(6):1087–1092

    Article  CAS  Google Scholar 

  • Hertog MG, Feskens EJ, Hollman PC, Katan MB, Kromhout D (1993) Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen elderly study. Lancet 342:1007–1011

    Article  CAS  Google Scholar 

  • Hoper L, Cassidy A (2006) A review of the health care potential of bioactive compounds. J Sci Food Agric 86:1805–1813

    Article  Google Scholar 

  • Imeh U, Khokhar S (2002) Distribution of conjugated and free phenols in fruits: antioxidant activity and cultivar variations. J Agr Food Chem 50(22):6301–6306

    Article  CAS  Google Scholar 

  • Jayaprakasha GK, Singh RP, Sakariah KK (2002) Antioxidant activity of grape seed (vitis vinifera) extracts on peroxidation models in vitro. Food Chem 73(3):285–290

    Google Scholar 

  • Loizzo MR, Said A, Tundis R, Hawas UW, Rashed K, Menichini F, Frega NG, Menichini F (2009) Antioxidant and antiproliferative activity of Diospyros lotus L. extract and isolated compounds. Plant Foods Hum Nutr 64(4):264–270

    Article  CAS  Google Scholar 

  • Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Fazelian M, Eslami B (2009) In vitro Antioxidant and Free Radical Scavenging Activity of Diospyros lotus and Pyrus boissieriana growing in Iran. Phcog Mag 5(18):122–126

    Google Scholar 

  • Othman A, Ismail A, Ghani NA, Adenan I (2007) Antioxidant capacity and phenolic content of cocoa beans. Food Chem 100(4):1523–1530

    Article  CAS  Google Scholar 

  • Ramakrishnan K, Narayanan P, Vasudevan V, Muthukumaran G, Antony U (2010) Nutrient composition of cultivated stevia leaves and the influence of polyphenols and plant pigments on sensory and antioxidant properties of leaf extracts. J Food Sci Tech 47(1):27–33

    Article  Google Scholar 

  • Singh N, Rajini PS (2004) Free radical scavenging activity of an aqueous extract of potato peel. Food Chem 85:611–616

    Article  CAS  Google Scholar 

  • Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult 16:144–153

    CAS  Google Scholar 

  • Soares JR, Dins TCP, Cunha AP, Almeida LM (1997) Antioxidant activity of some extracts of Thymus zygis. Free Radic Res 26(5):469–478

    Article  CAS  Google Scholar 

  • Srivastava A, JaganMohanRao L, Shivanandappa T (2007) Isolation of ellagic acid from the aqueous extract of the roots of Decalepis hamiltonii: antioxidant activity and cytoprotective effect. Food Chem 103(1):224–233

    Article  CAS  Google Scholar 

  • Su L, Yin JJ, Charlesc D, Zhoua K, Moorea J, Yu LL (2007) Total phenolic contents, chelating capacities and radical-scavenging properties of black peppercorn, nutmeg, rosehip, cinnamon and oregano leaf. Food Chem 100(3):990–997

    Article  CAS  Google Scholar 

  • Sun T, Powers JR, Tang JM (2007) Evaluation of the antioxidant activity of asparagus, broccoli and their juices. Food Chem 105(1):101–106

    Article  CAS  Google Scholar 

  • Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44–84

    Article  CAS  Google Scholar 

  • Wang J, Sun B, Cao Y (2007) Optimisation of ultrasound-assisted extraction of phenolic compounds from wheat bran. Food Chem 106:804–810

    Article  Google Scholar 

  • Yildirim A, Mavi A, Kara A (2001) Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J Agric Food Chem 49(8):4083–4089

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Wei Cao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gao, H., Cheng, N., Zhou, J. et al. Antioxidant activities and phenolic compounds of date plum persimmon (Diospyros lotus L.) fruits. J Food Sci Technol 51, 950–956 (2014). https://doi.org/10.1007/s13197-011-0591-x

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13197-011-0591-x

Keywords

Navigation