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Antimicrobial properties of chitosan nanoparticles: Mode of action and factors affecting activity

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Abstract

The present investigation describes the synthesis and characterization of novel biodegradable nanoparticles based on chitosan for biomedical applications. The presence of primary amine groups in repeating units of chitosan grants it several properties like antibacterial activity, antitumor activity and so on. Chitosan forms nanoparticles spontaneously on the addition of polyanion tripolyphosphate which has greater antimicrobial activity than parent chitosan. In the present study, chitosan nanoparticles (ChNP) were prepared by the ionic gelation method. The physiochemical characteristics of nanoparticles were analyzed using XRD, SEM, FTIR. The antibacterial activity of chitosan nanoparticles against medical pathogens Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa was evaluated by calculation of minimum inhibitory concentration (MIC) and compared with chitosan and chitin activity. The mode of action and factors affecting antibacterial activity were also analyzed. ChNP compounds exhibited superior antimicrobial activity against all microorganisms in comparison with chitosan and chitin. The antibiofilm activity was studied using crystal violet assay and growth on congo red agar. The study is thus a good demonstration of the applicability of chitosan nanoparticles as an effective antimicrobial agent with antibiofilm activity as well.

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References

  1. M. Raffi, S. Mehrwan, T. M. Bhatti, J. I. Akhter, A. Hameed, W. Yawar, and M. M. Ul Hasan, Ann. Microbiol., 60, 75 (2010).

  2. M. S. Usman, M. E. El Zowalaty, K. Shameli, N. Zainuddin, M. Salama, and N. A. Ibrahim, Int. J. Nanomed., 8, 4467 (2013).

    Google Scholar 

  3. L. Didenko, D. Gerasimenko, N. Konstantinova, T. Silkina, I. Avdienko, G. Bannikova, and V. Varlamov, B Exp. Biol. Med., 140, 356 (2005).

    Article  CAS  Google Scholar 

  4. M. E. I. Badawy and E. I. Rabea, Int. J. Carbohydr. Chem., 2011, 1 (2011).

    Article  Google Scholar 

  5. S. Pangburn, P. Trescony, and J. Heller, Biomaterials, 3, 105 (1982).

    Article  CAS  Google Scholar 

  6. S. B. Rao and C. P. Sharma, J. Biomed. Mater. Res., 34, 21 (1997).

    Article  CAS  Google Scholar 

  7. S. K. Kim and N. Rajapakse, Carbohydr. Polym., 62, 357 (2005).

    Article  CAS  Google Scholar 

  8. Y. Okamoto, S. Minami, A. Matsuhashi, H. Sashiwa, H. Saimoto, Y. Shigemasa, T. Tanigawa, Y. Tanaka, and S. Tokura, J. Vet. Med. Sci., 55, 743 (1993).

    Article  CAS  Google Scholar 

  9. M. Thongngam and D. J. McClements, J. Agri. Food Chem., 52, 987 (2004).

    Article  CAS  Google Scholar 

  10. I. Helander, E. L. Nurmiaho-Lassila, R. Ahvenainen, J. Rhoades, and S. Roller, Int. J. Food Microbiol., 71, 235 (2001).

    Article  CAS  Google Scholar 

  11. P. J. Park, J. Y. Je, and S. K. Kim, Carbohydr. Polym., 55, 17 (2004).

    Article  CAS  Google Scholar 

  12. Z. X. Tang, J. Q. Qian, and L. E. Shi, Appl. Biochem. Biotechnol., 136, 77 (2007).

    Article  CAS  Google Scholar 

  13. L. Yien, N. M. Zin, A. Sarwar, and H. Katas, Int. J. Biomater, 2012, 1 (2012).

    Google Scholar 

  14. L. E. C. de Paz, A. Resin, K. A. Howard, D. S. Sutherland, and P. L. Wejse, Appl. Environ. Microbiol., 77, 3892 (2011).

    Article  Google Scholar 

  15. L. Huang, X. Cheng, C. Liu, K. Xing, J. Zhang, G. Sun, X. Li, and X. Chen, Front. Biol., 4, 321 (2009).

    Article  Google Scholar 

  16. P. Kaur, A. Choudhary, and R. Thakur, Int. J. Sci. Eng. Res., 4, 869 (2013).

    Google Scholar 

  17. L. Qi, Z. Xu, X. Jiang, C. Hu, and X. Zou, Carbohydr. Res., 339, 2693 (2004).

    Article  CAS  Google Scholar 

  18. P. Kaur, R. Thakur, and A. Choudhary, Int. J. Sci. Technol., Res., 1, 83 (2012).

    Google Scholar 

  19. E. Russo, N. Gaglianone, S. Baldassari, B. Parodi, S. Cafaggi, C. Zibana, M. Donalisio, V. Cagno, D. Lembo, and G. Caviglioli, Colloid Surf. B-Biointerfaces, 118, 117 (2014).

    Article  CAS  Google Scholar 

  20. Y. Mori, T. Ono, Y. Miyahira, V. Q. Nguyen, T. Matsui, and M. Ishihara, Nanoscale Res. Lett., 8, 88 (2013).

    Article  Google Scholar 

  21. Y. C. Chung, Y. P. Su, C. C. Chen, G. Jia, H. I. Wang, J. G. Wu, and J. G. Lin, Acta Pharmacol. Sin., 25, 932 (2004).

    CAS  Google Scholar 

  22. X. Wang, Y. Du, and H. Liu, Carbohydr. Polym., 56, 21 (2004).

    Article  CAS  Google Scholar 

  23. Z. Zhong, R. Xing, S. Liu, L. Wang, S. Cai, and P. Li, Carbohydr. Res., 343, 566 (2008).

    Article  CAS  Google Scholar 

  24. A. Nagy, A. Harrison, S. Sabbani, R. S. Munson, P. K. Dutta, and W. J. Waldman, Int. J. Nanomed., 6, 1833 (2011).

    CAS  Google Scholar 

  25. R. Cuero, G. Osuji, and A. Washington, Biotechnol. Lett., 13, 441 (1991).

    Article  CAS  Google Scholar 

  26. M. Kong, X. G. Chen, K. Xing, and H. Park, J. Int. J. Food Microbiol., 144, 51 (2010).

    Article  CAS  Google Scholar 

  27. P. Calvo, C. R. Lopez, J. L. V. Jato, and M. J. Alonso, J. Appl. Polym. Sci., 63, 125 (1997).

    Article  CAS  Google Scholar 

  28. J. A. J. Nesalin and A. A. Smith, Asian J. Pharm. Sci., 7, 80 (2012).

    Google Scholar 

  29. A. Sarwar, H. Katas, and N. M. Zin, J. Nanopart. Res., 16, 1 (2014).

    Article  CAS  Google Scholar 

  30. S. H. Moussa, A. A. Tayel, A. A. Al-Hassan, and A. Farouk, J. Mycol., 2013, 2517 (2013).

    Google Scholar 

  31. A. Tarafdar and G. Biswas, Int. J. Theor. Appl. Res. Mech. Eng., 2, 2319 (2013).

    Google Scholar 

  32. K. H. Prashanth, F. Kittur, and R. Tharanathan, Carbohydr. Polym., 50, 27 (2002).

    Article  Google Scholar 

  33. H. Katas and H. O. Alpar, J. Control Release, 115, 216 (2006).

    Article  CAS  Google Scholar 

  34. J. Kumirska, M. Czerwicka, Z. Kaczynski, A. Bychowska, K. Brzozowski, J. Thoming, and P. Stepnowski, Mar. Drugs, 8, 1567 (2010).

    Article  CAS  Google Scholar 

  35. M. T. Yen, J. H. Yang, and J. L. Mau, Carbohydr. Polym., 75, 15 (2009).

    Article  CAS  Google Scholar 

  36. X. Yin, X. Zhang, Q. Lin, Y. Feng, W. Yu, and Q. Zhang, Arkivoc, 9, 66 (2004).

    Google Scholar 

  37. K. C. A. G. Annadurai, J. Acad. Ind. Res., 1, 4 (2012).

    Google Scholar 

  38. Y. Xu and Y. Du, Int. J. Pharm, 250, 215 (2003).

    Article  CAS  Google Scholar 

  39. U. K. Parida, N. Rout, and B. K. Bindhani, Adv. Biosci. Biotechnol., 2013, 1 (2013).

    Google Scholar 

  40. C. Z. Chen and S. L. Cooper, Biomaterials, 23, 3359 (2002).

    Article  CAS  Google Scholar 

  41. S. K. R. Namasivayam and E. A. Roy, Int. J. Sci. Res. Publi., 3, 591 (2013).

    Google Scholar 

  42. A. Hassan, J. Usman, F. Kaleem, M. Omair, A. Khalid, and M. Iqbal, Braz. J. Infect. Dis., 15, 305 (2011).

    Article  Google Scholar 

  43. S. Moteeb, Journal of University of Anbar for Pure Science, 2, 3 (2008).

    Google Scholar 

  44. L. E. C. de Paz, A. Resin, K. A. Howard, D. S. Sutherland, and P. L. Wejse, Appl. Environ. Microbiol., 77, 3892 (2011).

    Article  Google Scholar 

  45. K. Kalishwaralal, S. B. Manikanth, S. R. Pandian, V. Deepak, and S. Gurunath, Colloid Surf. B-Biointerfaces, 79, 340 (2010).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, 685560, India

    K. Divya, Smitha Vijayan, Tijith K. George & M. S. Jisha

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  1. K. Divya
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  2. Smitha Vijayan
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  3. Tijith K. George
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  4. M. S. Jisha
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Correspondence to M. S. Jisha.

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Divya, K., Vijayan, S., George, T.K. et al. Antimicrobial properties of chitosan nanoparticles: Mode of action and factors affecting activity. Fibers Polym 18, 221–230 (2017). https://doi.org/10.1007/s12221-017-6690-1

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  • DOI: https://doi.org/10.1007/s12221-017-6690-1

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