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Overexpression of long noncoding RNA PCAT-1 is a novel biomarker of poor prognosis in patients with colorectal cancer

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Abstract

Long noncoding RNAs (lncRNA) are emerging as key molecules in human cancer. Prostate cancer–associated ncRNA transcripts 1 (PCAT-1), a lncRNA, has been recently revealed involving in human prostate cancer progression. However, whether PCAT-1 could serve as novel biomarker to predict prognosis in colorectal cancer (CRC) or not is unknown. We therefore carried out the present study to explore the correlation between PCAT-1 expression and the progression of CRC. In this study, the expression of PCAT-1 in 108 cases of CRC tissues and matched 81 adjacent normal tissues were determined by quantitative real-time PCR. Furthermore, the copy number variation of PCAT-1 was also measured in 17 tumor tissues and matched normal tissues. Our results showed that PCAT-1 expression in CRC tissues was significantly upregulated compared with the matched normal tissues (p < 0.001) and the overexpression of PCAT-1(upregulated by more than 50 %) was found in 64 % (62/81) of CRC. Moreover, PCAT-1 gene copy number variation explains only a few percent of observed overexpression. In addition, there was a significant association between PCAT-1 expression and distant metastasis (p = 0.04), but not other clinical characteristics. More important, CRC patients with PCAT-1 higher expression have shown significantly poorer overall survival than those with lower PCAT-1 expression (p < 0.001). Also, multivariable Cox regression analysis identified PCAT-1 overexpression as an independent prognostic factor for CRC (p = 0.007, HR = 3.12 95 %CI = 1.355–7.185). In conclusion, our results suggest that high expression of PCAT-1 is involved in CRC progression and could be a novel biomarker of poor prognosis in patient with colorectal cancer.

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Acknowledgments

This work was supported by the National Basic Research Program of China (2011CB504303), the Ministry of Science and Technology of China (2011ZX09307-001-04), and the Natural Science Foundation of Guangdong Province (9151008004000002). We thank Dr. Guoping shen for comments on previous versions of this manuscript.

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The authors have no conflict of interest.

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Authors and Affiliations

  1. State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, China

    Xiaosong Ge, Yuanbin Chen, Xiaoyu Liao, Deqing Liu, Fangfang Li & Weihua Jia

  2. School of Public Health, Sun Yat-Sen University, Guangzhou, China

    Honglian Ruan

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  1. Xiaosong Ge
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  2. Yuanbin Chen
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  3. Xiaoyu Liao
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  4. Deqing Liu
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  7. Weihua Jia
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Correspondence to Weihua Jia.

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Ge, X., Chen, Y., Liao, X. et al. Overexpression of long noncoding RNA PCAT-1 is a novel biomarker of poor prognosis in patients with colorectal cancer. Med Oncol 30, 588 (2013). https://doi.org/10.1007/s12032-013-0588-6

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