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Immune-Related Antigens, Surface Molecules and Regulatory Factors in Human-Derived Mesenchymal Stromal Cells: The Expression and Impact of Inflammatory Priming

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An Erratum to this article was published on 13 October 2012

Abstract

Based on their ability to regulate immune responses, MSCs are considered to be potential candidates for managing immune-mediated diseases in the context of immune therapy. AT and WJ are considered valuable alternatives for BM as a source of MSCs. A detailed and comparative characterization of the immunological profile of MSCs derived from different sources, as well as an understanding of their responsiveness under certain circumstances, such as inflammation, is required to facilitate efficient and well-designed clinical studies. Flow cytometric analyses revealed clear differences among MSC types concerning the expression of the endothelial (e.g., CD31, CD34, CD144 and CD309) and stromal (e.g., CD90 and CD105) associated markers. Regardless of their source, MSCs did not express any of the known hematopoietic markers. All MSCs were uniformly positive for HLA-ABC and lacked the expression of HLA-DR and the co-stimulatory molecules (e.g., CD40, CD80, CD86, CD134 and CD252) required for full T-cell activation. Tissue-specific MSCs presented a modulated expression of cell adhesion molecules that is important for their cellular interactions. MSCs exhibited several surface (e.g., CD73, HLA-G, HO-1 and CD274) and soluble (e.g., HGF, PGE2 and IGFBP-3) immunoregulatory molecules. According to these immunological profiles, the present work provides evidence that the source from which MSCs are derived is important for the design of MSC-based immunointervention approaches. In light of these observations, we may suggest that WJ-MSCs appear to be the most attractive cell population to use in immune cellular therapy when immunosuppressive action is required.

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Abbreviations

AT:

Adipose tissue

BM:

Bone marrow

MSCs:

Mesenchymal stromal cells

WJ:

Wharton’s jelly

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Acknowledgments

M. Najar is a Télévie Scientific Research Worker of “Le Fonds National de la Recherche Scientifique” (FRS-FNRS 3.4.532.07F–7.4.524.08F). This study was also supported by BRUSTEM, an impulse program of “Institut d'encouragement de la Recherche Scientifique et de l'Innovation de Bruxelles” (IRSIB). The funding agencies did not play a role in the conduct of the research or the preparation of the manuscript; in particular, the funding agencies did not contribute to the study design, the collection, analysis and interpretation of data, the writing or the decision to submit the article.

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Authors and Affiliations

  1. Laboratory of Clinical Cell Therapy, Institut Jules Bordet, Université Libre de Bruxelles (ULB), Blvd de Waterloo no 121, 1000, Bruxelles, Brussels, Belgium

    Mehdi Najar, Gordana Raicevic, Cécile De Bruyn, Dominique Bron, Michel Toungouz & Laurence Lagneaux

  2. Laboratory of Experimental Hematology, Institut Jules Bordet, Université Libre de Bruxelles (ULB), Brussels, Belgium

    Hussein Fayyad Kazan & Dominique Bron

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  1. Mehdi Najar
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  2. Gordana Raicevic
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Correspondence to Mehdi Najar.

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Najar, M., Raicevic, G., Kazan, H.F. et al. Immune-Related Antigens, Surface Molecules and Regulatory Factors in Human-Derived Mesenchymal Stromal Cells: The Expression and Impact of Inflammatory Priming. Stem Cell Rev and Rep 8, 1188–1198 (2012). https://doi.org/10.1007/s12015-012-9408-1

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