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Effect of Dietary Vanadium on the Ileac T Cells and Contents of Cytokines in Broilers

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Abstract

The purpose of this 42-day study was to examine the effect of dietary vanadium on the ileac T cells and contents of cytokines including interleukin-2 (IL-2), interleukin-6 (IL-6), and interferon-gamma (IFN-γ) in broilers by flow cytometry and enzyme-linked immunosorbent assay. A total of 420 one-day-old avian broilers were divided into six groups (seven replicates in each group and ten broilers in each replicate) and fed on control diet or the same diet supplemented with 5, 15, 30, 45, and 60 mg/kg vanadium in the form of ammonium metavanadate. The results showed that the percentages of CD3+, CD3+CD4+, and CD3+CD8+ T cells in both ileac lamina propria lymphocytes (LPLs) and intraepithelial lymphocytes (IELs) were significantly lower (P < 0.05 or P < 0.01) in the 45- and 60-mg/kg groups than in the control group from 14 to 42 days of age. The CD4+/CD8+ ratio was increased in ileac LPLs in the 60-mg/kg group at 28 days of age, and in ileac IELs in the 60-mg/kg group at 28 days of age and in the 45-mg/kg group at 42 days of age. Meanwhile, the ileac IL-2, IL-6 contents were decreased (P < 0.05 or P < 0.01) in the 60-mg/kg group from 14 to 42 days of age and in the 45-mg/kg group from 28 to 42 days of age in comparison with those of the control group. It was concluded that dietary vanadium in excess of 30 mg/kg reduced the ileac T cell population and percentages of T cell subsets, and IL-2, IL-6, and IFN-γ contents, implying that the immune function of local intestinal mucosa in broilers could be affected by the dietary vanadium.

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Acknowledgments

The study was supported by the program for Changjiang Scholars and Innovative Research Team in University (IRT 0848) and the Education Department and Scientific Department of Sichuan Province (09ZZ017).

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Authors and Affiliations

  1. Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, 625014, China

    Kangping Wang, Hengmin Cui, Yuanxin Deng, Xi Peng, Jing Fang, Zhicai Zuo & Wei Cui

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  1. Kangping Wang
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  2. Hengmin Cui
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  3. Yuanxin Deng
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  4. Xi Peng
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  5. Jing Fang
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  6. Zhicai Zuo
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  7. Wei Cui
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Correspondence to Hengmin Cui.

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Kangping Wang and Yuanxin Deng contributed equally to this work and are considered as first authors.

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Wang, K., Cui, H., Deng, Y. et al. Effect of Dietary Vanadium on the Ileac T Cells and Contents of Cytokines in Broilers. Biol Trace Elem Res 147, 113–119 (2012). https://doi.org/10.1007/s12011-011-9274-8

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  • DOI: https://doi.org/10.1007/s12011-011-9274-8

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