Abstract
Biomass was pretreated with proton beam irradiation (PBI) in order to enhance enzyme digestibility. Rice straw and soaking in aqueous ammonia (SAA)-treated rice straw were treated with 1–25 kGy doses of PBI at a beam energy of 45 MeV. The optimal doses of PBI for efficient sugar recovery were 15 and 3 kGy for rice straw and SAA-treated rice straw, respectively. When PBI was applied to rice straw at 15 kGy, the glucose conversion reached 68% of the theoretical maximum at 72 h. When 3 kGy of PBI was applied to SAA-treated rice straw, approximately 90% of the theoretical glucose conversion was obtained at 12 h compared to a 89% conversion at 48 h. After 2 h of enzymatic saccharification, the initial reaction rates of raw rice straw pretreated with 15 kGy of PBI and SAA-treated rice straw pretreated with 3 kGy of PBI were 1.4 × 10−4 and 9.7 × 10−4 g L−1 s−1, respectively. Further, the results of X-ray diffractometry support the effect of PBI on sugar recovery, whereas scanning electron microscopy images revealed a more rugged rice straw surface.
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This research was supported by the Technology Development Program (309016-5) for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea, and funded by the Proton Engineering Frontier Project of the Korea Atomic Energy Research Institute (2008).
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Kim, S.B., Kim, J.S., Lee, J.H. et al. Pretreatment of Rice Straw by Proton Beam Irradiation for Efficient Enzyme Digestibility. Appl Biochem Biotechnol 164, 1183–1191 (2011). https://doi.org/10.1007/s12010-011-9204-4
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DOI: https://doi.org/10.1007/s12010-011-9204-4