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Ethanol Production from Residual Wood Chips of Cellulose Industry: Acid Pretreatment Investigation, Hemicellulosic Hydrolysate Fermentation, and Remaining Solid Fraction Fermentation by SSF Process

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Abstract

Current research indicates the ethanol fuel production from lignocellulosic materials, such as residual wood chips from the cellulose industry, as new emerging technology. This work aimed at evaluating the ethanol production from hemicellulose of eucalyptus chips by diluted acid pretreatment and the subsequent fermentation of the generated hydrolysate by a flocculating strain of Pichia stipitis. The remaining solid fraction generated after pretreatment was subjected to enzymatic hydrolysis, which was carried out simultaneously with glucose fermentation [saccharification and fermentation (SSF) process] using a strain of Saccharomyces cerevisiae. The acid pretreatment was evaluated using a central composite design for sulfuric acid concentration (1.0–4.0 v/v) and solid to liquid ratio (1:2–1:4, grams to milliliter) as independent variables. A maximum xylose concentration of 50 g/L was obtained in the hemicellulosic hydrolysate. The fermentation of hemicellulosic hydrolysate and the SSF process were performed in bioreactors and the final ethanol concentrations of 15.3 g/L and 28.7 g/L were obtained, respectively.

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Acknowledgments

The authors are deeply grateful to the Brazilian Council for Research (CNPq), the Rio de Janeiro State Foundation for Science & Technology (FAPERJ), and the Brazilian Oil Company (PETROBRAS) for grants and other financial supports.

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Authors and Affiliations

  1. Biochemical Engineering Department, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, 21945-970, Brazil

    Neumara Luci Conceição Silva, Gabriel Jaime Vargas Betancur, Edelvio de Barros Gomes & Nei Pereira Jr.

  2. Engineering Faculty–Chemical Engineering Department, University of Antioquia, Medellín, Colombia

    Mariana Peñuela Vasquez

Authors
  1. Neumara Luci Conceição Silva
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  2. Gabriel Jaime Vargas Betancur
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  3. Mariana Peñuela Vasquez
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  4. Edelvio de Barros Gomes
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  5. Nei Pereira Jr.
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Correspondence to Nei Pereira Jr..

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Silva, N.L.C., Betancur, G.J.V., Vasquez, M.P. et al. Ethanol Production from Residual Wood Chips of Cellulose Industry: Acid Pretreatment Investigation, Hemicellulosic Hydrolysate Fermentation, and Remaining Solid Fraction Fermentation by SSF Process. Appl Biochem Biotechnol 163, 928–936 (2011). https://doi.org/10.1007/s12010-010-9096-8

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  • DOI: https://doi.org/10.1007/s12010-010-9096-8

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