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In Vivo Evaluation of Cerebral Transplantation of Resovist-Labeled Bone Marrow Stromal Cells in Parkinson’s Disease Rats Using Magnetic Resonance Imaging

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Abstract

The effectiveness of Resovist-labeled bone marrow stem cells (BMSCs) was evaluated in vivo following their cerebral transplantation in a model of Parkinson’s disease (PD) in rats using MRI, and the MRI findings were further compared with the behavior and histopathological manifestations of these rats. Forty PD rats were randomly assigned into five groups according to the cell doses injected into the rat brain site: control group (normal saline injection) and groups injected with 1 × 105, 1.5 × 105, 2 × 105, and 2.5 × 105 BMSCs. Gradient echo T2-weighted images were obtained immediately after cell transplantation and repeatedly taken 1, 4, 8, and 12 week(s) after cell transplantation. The rotational behavior of the animals was observed before and 1, 4, and 8 week(s) after transplantation. The rats were killed after the last MRI scanning, the brain tissues were analyzed by histopathology techniques, and RNAs were extracted for the expression analysis of selected genes using RT-PCR. One week following cell transplantation, all injected sites showed well-defined hypointense areas on MR images, with the most significant effect observed in rats injected with 2 × 105 BMSCs. These MR findings in PD rats lasted up to 12 weeks. The effectiveness of BMSC transplantation revealed by MRI was well confirmed by the behavioral and histopathological observations as well as indirectly supported by gene expression analyses. With the use of SPIO labeling, MRI techniques provided a dynamic evaluation of the spatial and temporal changes following cell transplantation and allowed the association analysis among the imaging, functions, and gene expression analysis in rats. These data also suggest the therapeutic potential of transplanted BMSCs. It is reasonable to speculate that the use of MRI in in vivo evaluation of the effect and fate of transplanted cells in various disease models will be beneficial to developing new strategies of cell-based gene therapy.

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Abbreviations

BMSCs:

Bone marrow stem cells

MRI:

Magnetic resonance imaging

PD:

Parkinson’s disease

SPIO:

Superparamagnetic iron oxide

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Acknowledgments

This research was supported by the Natural science fund for colleges and universities in Jiangsu Province (no. 06KJB320097). Mr. Wenbing Li read this manuscript and gave some comments.

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Authors and Affiliations

  1. Department of Radiology, The Second Affiliated Hospital of Soochow University, Suzhou, China

    Jing Guo, Jun-Kang Shen, Jing Sun & Han Xu

  2. Department of Neurology, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China

    Lan Wang & Wei-Feng Luo

  3. Department of Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, China

    Li Xiao

  4. Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, China

    Rong-Jun Zhang & Zhi-Gang Gong

  5. IPS, Sherbrooke University Medical School, Quebec, Canada

    Pierre Sirois

  6. The Medical College of Soochow University, Suzhou, 215123, China

    Kai Li

Authors
  1. Jing Guo
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  2. Jun-Kang Shen
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  3. Lan Wang
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  4. Li Xiao
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  6. Wei-Feng Luo
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  7. Zhi-Gang Gong
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  8. Jing Sun
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  9. Han Xu
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  10. Pierre Sirois
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  11. Kai Li
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Corresponding authors

Correspondence to Jun-Kang Shen or Kai Li.

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Jing Guo and Jun-Kang Shen have contributed equally to this paper.

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Guo, J., Shen, JK., Wang, L. et al. In Vivo Evaluation of Cerebral Transplantation of Resovist-Labeled Bone Marrow Stromal Cells in Parkinson’s Disease Rats Using Magnetic Resonance Imaging. Appl Biochem Biotechnol 163, 636–648 (2011). https://doi.org/10.1007/s12010-010-9069-y

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  • DOI: https://doi.org/10.1007/s12010-010-9069-y

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