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Ellagic Acid Production from Biodegradation of Creosote Bush Ellagitannins by Aspergillus niger in Solid State Culture

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Abstract

The ability of Aspergillus niger GH1 in converting creosote bush ellagitannins into ellagic acid (EA) was evaluated in solid state culture. Creosote bush leaves were used to extract the ellagitannins fraction, which was impregnated in polyurethane foam used as support of solid state culture. Ellagitannins content, EA accumulation, and the related enzymatic activities were evaluated. A. niger GH1 was able to completely degrade creosote bush ellagitannins with an EA yield of 23.1% at 36 h of culture. The ability to degrade creosote bush ellagitannins exhibited by A. niger GH1 was clearly associated to an ellagitannin-hydrolysing enzyme with a maximum activity of 43 U/l, while that ability was not associated to tannase activity that was detected in the culture extract. This study demonstrated the great ability of A. niger GH1 to hydrolyze ellagitannins and the potential of solid state culture to produce the antioxidant EA by degradation of creosote bush ellagitannins.

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Acknowledgment

Financial support of this research was from the National Council of Science and Technology of Mexico with grant no. SEP-CONACYT-J-51360. Authors A. Aguilera-Carbo and J. S. Hernández-Rivera are graduate students supported by CONACYT, Mexico.

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Authors and Affiliations

  1. Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, 25280, Saltillo, Coahuila, Mexico

    Antonio Aguilera-Carbo, Juan S. Hernández & Cristóbal N. Aguilar

  2. IRD- Unité Biotrans, IMEP Case 441, Faculté des Sciences de Saint Jérôme, Université Paul Cézanne, Av. Escadrille Normandie-Niemen, 13397, Marseille Cedex 20, France

    Christopher Augur

  3. Department of Biotechnology, Universidad Autónoma Metropolitana, Iztapalapa, 09340, Federal District, Mexico

    Lilia A. Prado-Barragan & Ernesto Favela-Torres

Authors
  1. Antonio Aguilera-Carbo
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  2. Juan S. Hernández
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  3. Christopher Augur
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  4. Lilia A. Prado-Barragan
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  5. Ernesto Favela-Torres
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  6. Cristóbal N. Aguilar
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Correspondence to Cristóbal N. Aguilar.

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Aguilera-Carbo, A., Hernández, J.S., Augur, C. et al. Ellagic Acid Production from Biodegradation of Creosote Bush Ellagitannins by Aspergillus niger in Solid State Culture. Food Bioprocess Technol 2, 208–212 (2009). https://doi.org/10.1007/s11947-008-0063-0

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  • DOI: https://doi.org/10.1007/s11947-008-0063-0

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