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Sucrose regulated enhanced induction of anthraquinone, phenolics, flavonoids biosynthesis and activities of antioxidant enzymes in adventitious root suspension cultures of Morinda citrifolia (L.)

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An Erratum to this article was published on 22 September 2011

An Erratum to this article was published on 22 September 2011

Abstract

The effect of initial sucrose concentration was investigated in root suspension cultures of Morinda citrifolia to improve root growth and secondary metabolites production, i.e. anthraquinone, phenolics and flavonoids. Besides, oxidative stress level, antioxidant enzymes activity and membranes damage under different sucrose concentration were estimated. A 5% sucrose supply was shown to be optimal for the production of root dry mass, but higher sucrose concentrations of 7–9% inhibited the accumulation of root dry weight (DW). However, the maximum production of anthraquinone (251.89 g L−1 DW), phenolics (165.14 g L−1 DW) and flavonoids (163.56 g L−1 DW) were achieved at 1% sucrose-treated culture, which may be a source carbon skeletons for secondary metabolism. At the same time was observed low oxidative damage, which could be associated with high levels of secondary metabolites and the increased activity of catalase. Although, catalase (CAT) activity were stimulated at 7–9% sucrose-treated cultures, high accumulation of hydrogen peroxide (H2O2) and peroxidation of lipid (MDA) was induced. The observed high activity of CAT and guaiacol peroxidase (G-POD) were not sufficient enough to mitigate the toxic effect of H2O2.

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Abbreviations

AQ:

Anthraquinone

CAT:

Catalase

DPPH:

1,1-Diphenyl-2-picrylhydrazyl

G-POD:

Guaiacol peroxidase

IBA:

Indole butyric acid

MDA:

Malondialdehyde

MS:

Murashige and Skoog

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Authors and Affiliations

  1. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    Md. Abdullahil Baque, Eun-Jung Lee & Kee-Yoeup Paek

  2. Department of Agronomy, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Md. Abdullahil Baque

  3. Center of Excellence in Biotechnology Research, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

    Abdullah Elgirban

  4. CBN PLANTECH Co. Ltd., Industry Academic Cooperation Foundation Agribusiness Incubator Center 206, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    Eun-Jung Lee

Authors
  1. Md. Abdullahil Baque
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  2. Abdullah Elgirban
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  3. Eun-Jung Lee
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  4. Kee-Yoeup Paek
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Corresponding author

Correspondence to Kee-Yoeup Paek.

Additional information

Communicated by M. Stobiecki.

An erratum to this article can be found at http://dx.doi.org/10.1007/s11738-011-0854-1.

An erratum to this article can be found at http://dx.doi.org/10.1007/s11738-011-0854-1.

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Baque, M.A., Elgirban, A., Lee, EJ. et al. Sucrose regulated enhanced induction of anthraquinone, phenolics, flavonoids biosynthesis and activities of antioxidant enzymes in adventitious root suspension cultures of Morinda citrifolia (L.). Acta Physiol Plant 34, 405–415 (2012). https://doi.org/10.1007/s11738-011-0837-2

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  • DOI: https://doi.org/10.1007/s11738-011-0837-2

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