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Novel PKCs activate ERK through PKD1 in MCF-7 cells

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Abstract

PKCs can have opposite effects on ERK phosphorylation. Novel (n)PKCs can inhibit ERK by phosphorylation of Raf-1, classical and atypical PKCs can activate ERK by removing an inhibitory protein from Raf-1. The aim of this work was to clarify how PMA-activated PKCs lead to ERK activation in MCF-7 cells expressing mainly nPKCs. Using chemical inhibitors and antibodies against PKCs, delivered into cells by the Chariot transfection system, we found that nPKCs activate ERK through transphosphorylation of PKD1, the blockage of which prevented PMA-stimulated ERK activation. We conclude that the nPKCs/PKD1 cascade is determinant for ERK activation by PMA in MCF-7 cells.

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Acknowledgments

This work was supported by project grants from the Italian Ministero dell’Università e della Ricerca (MIUR, ex 60%).

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Authors and Affiliations

  1. Department of Physiology, University of Siena, via Aldo Moro, 7, 53100, Siena, Italy

    Claudia Torricelli & Emanuela Maioli

  2. Department of Biomedical Science, University of Siena, via Aldo Moro, 7, 53100, Siena, Italy

    Giuseppe Valacchi

  3. Department of Food and Nutrition, Kyung Hee University, Seoul, South Korea

    Giuseppe Valacchi

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  1. Claudia Torricelli
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  2. Giuseppe Valacchi
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  3. Emanuela Maioli
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Correspondence to Emanuela Maioli.

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Editor: J. Denry Sato

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Torricelli, C., Valacchi, G. & Maioli, E. Novel PKCs activate ERK through PKD1 in MCF-7 cells. In Vitro Cell.Dev.Biol.-Animal 47, 73–81 (2011). https://doi.org/10.1007/s11626-010-9355-6

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  • DOI: https://doi.org/10.1007/s11626-010-9355-6

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