Abstract
Actinomycetes from less explored ecosystems were screened for antibacterial and antimycobacterial activity. Crude bioactive compounds were produced by growing these strains by shake flask fermentation using soybean meal medium. Culture supernatant and mycelia were extracted with ethyl acetate and methanol, respectively. Antibacterial activity of crude extracts was tested by disc diffusion method against gram positive and gram negative bacteria. Actinomycete strains D10, D5, NEK5, ANS2, M104 and R2 showed prominent activity. Culture filtrates and crude extracts were tested against standard strain Mycobacterium tuberculosis H37Rv and drug sensitive and drug resistant clinical isolates of M. tuberculosis by luciferase reporter phage (LRP) assay. Considerable variation was observed in antimycobacterial activity between actinomycete culture filtrates and solvent extracts. Actinomycete strains viz., D10, D5 (desert), CSA14 (forest), CA33 (alkaline soil), NEK5 (Neem plant), MSU, ANS2, R2 and M104 (marine) screened in the present study were found to be highly potent showing good antibacterial and antimycobacterial activity. Five of them such as A3, CSA1, EE9, ANS5 and R9 were exclusively active against M. tuberculosis. Secretary products of actinomycetes of rare ecosystems are meant to antagonize organisms in their respective environments. These are likely to be novel antimycobacterial compounds as they unknown to human pathogens.
This is a preview of subscription content, log in via an institution to check access.
References
Augustine SK, Bhavsar SP, Kapadnis BP (2005) Production of a growth dependent metabolite active against dermatophytes by Streptomyces rochei AK 39. Indian J Med Res 121:164–170
Berdy J (2005) Bioactive microbial metabolites. J Antibiot (Tokyo) 58:1–26
Busti E, Monciardini P, Cavaletti L, Bamonte R, Lazzarini A, Sosio M, Donadio S (2006) Antibiotic-producing ability by representatives of a newly discovered lineage of actinomycetes. Microbiol 152:675–683
Casenghi M, Cole ST, Nathan CF (2007) New approaches to filling the gap in tuberculosis drug discovery. PLoS Med 4:e293
De Souza MV (2006) Promising drugs against tuberculosis. Recent Pat Antiinfect Drug Discov 1:33–44
El Sayed KA, Bartyzel P, Shen X, Perry TL, Zjawiony JK, Hamann MK (2000) Marine natural products as antituberculosis agents. Tetrahedron 56:949–953
Franco MM, Countinho LEL (1991) Detection of novel secondary metabolites. Crit Rev Microbiol 11:193–276
Kobarfard F (2004) Tuberculosis and traditional medicine: fighting the oldest infectious disease using the oldest source of medicine. Iranian J Phar Res 2:71–72
Lam KS (2006) Discovery of novel metabolites from marine actinomycetes. Curr Opin Microbiol 9:245–251
Nonomura H (1974) Key for classification and identification of 458 species of the streptomycetes included in ISP. J Ferment Technol 52(2):78–92
Radhakrishnan M, Balaji S, Balagurunathan R (2007) Thermotolerant actinomycetes from the Himalayan mountain-antagonistic potential, characterization and identification of selected strains. Malays Appl Biol 36(1):59–65
Riska PF, Su Y, Bardarov S, Freundlich L, Sarkis G, Hatfull G, Carriere C, Kumar V, Chan J, Jacobs WR Jr (1999) Rapid film-based determination of antibiotic susceptibilities of Mycobacterium tuberculosis strains by using a luciferase reporter phage and the Bronx Box. J Clin Microbiol 37:1144–1149
Saha M, Jaisankar P, Das J, Sarkar KK, Roy S, Bersa SB, Vedasiromani JR, Ghosh D, Sana B, Mukherjee J (2006) Production and purification of bioactive substance inhibiting multiple drug resistant bacteria and human leukemia cells from a salt-tolerant marine Actinobacterium sp. isolated from the Bay of Bengal. Biotechnol Lett 28:1083–1088
Shirling EB, Gottileb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340
Sivakumar PM, Seenivasan SP, Kumar V, Doble M (2007) Synthesis, antimycobacterial activity evaluation, and QSAR studies of chalcone derivatives. Bioorg Med Chem Lett 17:1695–1700
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Radhakrishnan, M., Suganya, S., Balagurunathan, R. et al. Preliminary screening for antibacterial and antimycobacterial activity of actinomycetes from less explored ecosystems. World J Microbiol Biotechnol 26, 561–566 (2010). https://doi.org/10.1007/s11274-009-0198-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11274-009-0198-9