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Actinomycetes from Moroccan habitats: isolation and screening for cytotoxic activities

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Abstract

In our screening for actinomycetes showing cytotoxic activities, 8 samples were collected from various Moroccan habitats, 136 isolates were tested for their capacity to produce antibacterial compounds against gram positive bacteria. Thirty-seven strains of these isolates were active against Gram-positive bacteria. Using the following steps of primary screening: antibacterial activity, confrontation between the isolates and toxicity to Artemia salina; fifteen different isolates were used for further investigation. The aqueous extracts of Streptomyces sp. T5 and Streptomyces sp. AS8 were selected for their cytotoxic activity against Hep2, BSR and P815 cell lines, and two active compounds were observed on HPLC. The two isolates exhibited high activity against human cancer cell lines and were inactive on PBMC cell lines. Furthermore, the Streptomyces sp. T5 extract showed a proliferative activity.

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Acknowledgements

The authors wish to thank Prof. A. Benharref, L. Ait M’Barek and A. Boussaid for their help. The selected isolates were identified by Institut Pasteur Paris France.

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Authors and Affiliations

  1. Département de Biologie, Faculté des Sciences Semlalia, Laboratoire de Biologie et Biotechnologie des Microorganismes, Marrakech, Morocco

    M. Anibou & Y. Ouhdouch

  2. Département de Biologie, Faculté des Sciences Semlalia, Laboratoire de Pharmacologie Neurobiologie et Comportement, Marrakech, Morocco

    M. Anibou & A. Chait

  3. Faculté des Sciences et Techniques, Laboratoire d’Immunologie Biochimie et Biologie Moléculaire, Equipe des Substances Naturelles et Immunopharmacologie cellulaire et moléculaire, Beni Mellal, Morocco

    A. Zyad

  4. Laboratoire de Chimie bio-organique et macromoleculaire FST Guéliz, Marrakech, Morocco

    M. Taourirt

  5. Département de Faculté des Sciences Semlalia, Laboratoire de Chimie Organique des Heterocycles, Marrakech, Morocco

    A. Benherref

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  1. M. Anibou
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  2. A. Chait
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  3. A. Zyad
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  4. M. Taourirt
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  5. Y. Ouhdouch
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  6. A. Benherref
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Correspondence to Y. Ouhdouch.

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Anibou, M., Chait, A., Zyad, A. et al. Actinomycetes from Moroccan habitats: isolation and screening for cytotoxic activities. World J Microbiol Biotechnol 24, 2019–2025 (2008). https://doi.org/10.1007/s11274-008-9705-7

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  • DOI: https://doi.org/10.1007/s11274-008-9705-7

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