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Decolorization of textile dyes by enzymatic extract and submerged cultures of Pleurotus sajor-caju

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Abstract

Pleurotus sajor-caju PS2001 was screened in Petri dish plates to assess the dye-decolorizing ability of industrial textile dyes. P. sajor-caju PS2001 was also cultivated in solid-state fermentation containing sawdust of Pinus sp. and wheat bran to obtain the enzymatic extract, showing laccase and manganese-peroxidase activity, which was used to test the capacity to degrade the textile dyes. Additional tests of decolorization were performed in liquid cultures. Anthraquinone-type textile dyes proved to be substrates for the enzymatic system of P. sajor-caju PS2001. Cultures in Petri dish plates showed that the anthraquinone dye Reactive Blue 220 can act as a redox mediator for the enzymatic reactions involved in the decolorization process, and enables the azo dye degradation. Reactive Blue 220 and Acid Blue 280 were completely decolorized in 30 min and 60 min, respectively, during the tests with precipitated enzymatic extract, while the azo dyes showed resistance to degradation. Additionally, in submerged cultures with dyes, veratryl alcohol oxidases and lignin peroxidase activities were observed. These results suggest that the strain P. sajor-caju PS2001 has great potential for use in the bioremediation technology of recalcitrant pollutant such as textile effluents.

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Acknowledgments

This work was supported by research grants from FAPERGS (Fundação de Amparo à Pesquisa do Rio Grande do Sul) and UCS (Universidade de Caxias do Sul). F. M. Munari received a fellowship from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior).

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  1. Instituto de Biotecnologia, Universidade de Caxias do Sul, Rua Francisco Getúlio Vargas, 1130, Caixa Postal 1352, Caxias do Sul, RS, 95070-560, Brazil

    Fernanda M. Munari, Tamara A. Gaio, Raquel Calloni & Aldo J. P. Dillon

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  1. Fernanda M. Munari
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  2. Tamara A. Gaio
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  3. Raquel Calloni
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  4. Aldo J. P. Dillon
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Correspondence to Aldo J. P. Dillon.

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Munari, F.M., Gaio, T.A., Calloni, R. et al. Decolorization of textile dyes by enzymatic extract and submerged cultures of Pleurotus sajor-caju . World J Microbiol Biotechnol 24, 1383–1392 (2008). https://doi.org/10.1007/s11274-007-9621-2

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  • DOI: https://doi.org/10.1007/s11274-007-9621-2

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