Abstract
Lipase from the oil-mill waste isolate Cunninghamella verticillata was purified by stepwise precipitation using acetone, as a sequel to our earlier conventional column chromatographic method [Gopinath et al. (2002)World Journal of Microbiology and Biotechnology 18, 449–458]. The yield of purified lipase was approx. 4-fold higher than by the previous method and the purified lipase was obtained with 70–80% acetone saturations. The enzyme was resolved as a single band with homogeneity both by native and by SDS–PAGE. The optimum condition for the lipase to crystallize was 5 μg of enzyme in 0.05 M sodium phosphate buffer (pH 6.5) with 5 mM FeCl2 and 10% 2-methyl 2,4-pentanediol (MPD).
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Kumarevel, T.S., Gopinath, S.C.B., Hilda, A. et al. Purification of lipase from Cunninghamella verticillata by stepwise precipitation and optimized conditions for crystallization. World J Microbiol Biotechnol 21, 23–26 (2005). https://doi.org/10.1007/s11274-004-1005-2
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DOI: https://doi.org/10.1007/s11274-004-1005-2