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Characterization of T-DNA Insertion Mutants and RNAi Silenced Plants of Arabidopsis thaliana UV-damaged DNA Binding Protein 2 (AtUV-DDB2)

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Abstract

The human UV-damaged DNA binding protein (UV-DDB), a heterodimeric protein composed of 127 kDa (UV-DDB1) and 48 kDa (UV-DDB2) subunits, has been shown to be involved in DNA repair. To elucidate the in vivo function of plant UV-DDB2, we have analyzed T-DNA insertion mutants of the Arabidopsis thaliana UV-DDB2 subunit (atuv-ddb2 mutants) and AtUV-DDB2 RNAi silenced plants (atuv-ddb2 silenced plants). atuv-ddb2 mutants and atuv-ddb2 silenced plants were both viable, suggesting that AtUV-DDB2 is not essential for survival. Interestingly, both plant types showed a dwarf phenotype, implying impaired growth of the meristem. To the best of our knowledge, this is the first occasion that a dwarf phenotype has been found to be associated with a UV-DDB2 mutation in either plants or animals. The mutants also demonstrated increased sensitivity to UV irradiation, methyl methanesulfonate and hydrogen peroxide treatment, indicating that AtUV-DDB2 is also involved in DNA repair. Our results lead us to suggest that not only does AtUV-DDB2 function in DNA repair, it also has a direct or indirect influence on cell proliferation in the plant meristem.

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Authors and Affiliations

  1. Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510, Noda, Chiba, Japan

    Asami Koga, Toyotaka Ishibashi, Seisuke Kimura, Yukinobu Uchiyama & Kengo Sakaguchi

  2. Ausio Laboratory, Department of Biochemical and Microbiology, University of Victoria, Victoria, BC, Canada

    Toyotaka Ishibashi

  3. Section of Plant Biology, 1002 Life Science Addition, University of California, Davis, CA, 95616, USA

    Seisuke Kimura

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  1. Asami Koga
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  2. Toyotaka Ishibashi
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  3. Seisuke Kimura
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  4. Yukinobu Uchiyama
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  5. Kengo Sakaguchi
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Correspondence to Kengo Sakaguchi.

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Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries.

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Koga, A., Ishibashi, T., Kimura, S. et al. Characterization of T-DNA Insertion Mutants and RNAi Silenced Plants of Arabidopsis thaliana UV-damaged DNA Binding Protein 2 (AtUV-DDB2). Plant Mol Biol 61, 227–240 (2006). https://doi.org/10.1007/s11103-006-6408-z

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