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Effects of Photodynamic Therapy on the Growth and Antifungal Susceptibility of Scedosporium and Lomentospora spp.

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Abstract

Scedosporium and Lomentospora species are the second most frequent colonizing, allergenic, or invasive fungal pathogens in patients with cystic fibrosis, and are responsible for infections varying from cutaneous and subcutaneous tissue infections caused by traumatic inoculation to severe systemic diseases in immunocompromised patients. The clinical relevance of fungal airway colonization for individual patients harboring Scedosporium and Lomentospora species is still an underestimated issue. The high resistance of Scedosporium and Lomentospora species to antifungal drugs has highlighted the need for alternative treatment modalities, and antimicrobial photodynamic therapy may be one such alternative. In this study, methylene blue was applied as a photosensitizing agent to 6 type strains of Scedosporium and Lomentospora species, and we irradiated the strains using a light-emitting diode (635 ± 10 nm, 12 J/cm2). We evaluated the effects of photodynamic therapy on strain growth and on the in vitro susceptibility of the strains to itraconazole, voriconazole, posaconazole, and amphotericin B. A colony-forming unit reduction of up to 5.2 log10 was achieved. Minimal inhibitory concentration ranges also decreased significantly with photoinactivation. Photodynamic therapy improved both the inactivation rates and the antifungal susceptibility profile of all fungal isolates tested.

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Acknowledgements

We gratefully acknowledge Professor Sybren de Hoog from the Centraalbureau voor Schimmelcultures Fungal Biodiversity Centre (CBS), Utrecht, The Netherlands, for kindly providing us with the strains studied. In addition, we thank Professor Elena Piecková from Slovak Medical University, Slovakia, for excellent guidance with regard to the data analysis.

Funding

This work was supported by the National Natural Science Foundation of China (81401713 to Qiaoyun Lu, 31400131 to Lujuan Gao, and 81401677 to Yi Sun), the Outstanding Youth Project of Zhongshan Hospital, Fudan University (2015ZSYXQN21 to Lujuan Gao), and the Hubei Provincial Health and Family Planning Scientific Research Project (WJ2015MB281 to Yi Sun).

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Author notes

  1. Qiaoyun Lu and Yi Sun contributed equally to this study and are joint first authors.

Authors and Affiliations

  1. Department of Dermatology, Central Hospital of Xiangyang, Hubei College of Arts and Science, No. 136 Jingzhou Street, Xiangcheng District, Xiangyang, 441021, People’s Republic of China

    Qiaoyun Lu & Shoubao Xiang

  2. Department of Dermatology, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, 434100, People’s Republic of China

    Yi Sun

  3. Department of Dermatology, Hospital of Wuhan University, Wuhan, 430000, People’s Republic of China

    Dingdan Tian

  4. Department of Dermatology, Zhongshan Hospital, Fudan University, No. 180 Fenglin Road, Xuhui District, Shanghai, 200032, People’s Republic of China

    Lujuan Gao

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  1. Qiaoyun Lu
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  2. Yi Sun
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Correspondence to Qiaoyun Lu or Lujuan Gao.

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Lu, Q., Sun, Y., Tian, D. et al. Effects of Photodynamic Therapy on the Growth and Antifungal Susceptibility of Scedosporium and Lomentospora spp.. Mycopathologia 182, 1037–1043 (2017). https://doi.org/10.1007/s11046-017-0195-8

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  • DOI: https://doi.org/10.1007/s11046-017-0195-8

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