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Enhancement of Candida albicans Virulence After Exposition to Cigarette Mainstream Smoke

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Abstract

The habit of cigarette smoking is associated with higher oral candidal carriage and possible predisposition to oral candidosis. The effects of exposure to smoke on the virulence properties of oral yeasts remain obscure. Hence, we showed in vitro the effect of cigarette smoke condensate (CSC) on ten clinical isolates of Candida albicans obtained from nonsmoking volunteers, as well the type-strain CBS562. CSC was generated by complete burn of five commercial cigarettes in an in-house smoking machine and used to prepare the culture broth in which the strains were grown. In 24-h intervals (T24, T48, and T72), the cells were harvested, washed, subcultured, and the resultant growth were evaluated for possible variations for secreted aspartyl protease, phospholipase, chondroitinase, and hemolysins, adhesion to acrylic and cell surface hydrophobicity (CSH). The results indicated a temporal increase in the secretion rates of enzymes, particularly when yeast cells were exposed to CSC for 48–72 h (P < 0.05). Similarly, adhesion to acrylic and CSH increased with exposure period (P < 0.05). Based on foregoing, we concluded that CSC may promote significant enhance in the secretion of candidal histolytic enzymes and adherence to denture surfaces, thereby promoting oral yeast carriage and possible infection.

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Acknowledgments

This study was conducted using intramural funds from The Pontifical Catholic University of Paraná (PUCPR) and The University of Hong Kong (HKU). It was part of the Scientific Initiation Program of F·B.B. that was granted by the National Council of Technological and Scientific Development (CNPq). D.B. is a research fellow supported by the Scientific Initiation Program of PUCPR.

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Authors and Affiliations

  1. Laboratory of Stomatology, CCBS, Pontifical Catholic University of Paraná, Rua Imaculada Conceição 1155, Curitiba, 80215-901, Brazil

    Fernanda Brasil Baboni, Dayton Barp, Ana Claudia Santos de Azevedo Izidoro & Edvaldo Antonio Ribeiro Rosa

  2. Dental Clinics, General Hospital of Curitiba, Praça Marechal Alberto Ferreira de Abreu s/nº, Curitiba, 80420-020, Brazil

    Ana Claudia Santos de Azevedo Izidoro

  3. Oral Biosciences Unity, Prince Philip Dental Hospital, University of Hong Kong, 5F/34 Hospital Road, Hong Kong, Hong Kong SAR, China

    Lakshman Perera Samaranayake & Edvaldo Antonio Ribeiro Rosa

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  1. Fernanda Brasil Baboni
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  2. Dayton Barp
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Correspondence to Edvaldo Antonio Ribeiro Rosa.

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Baboni, F.B., Barp, D., de Azevedo Izidoro, A.C.S. et al. Enhancement of Candida albicans Virulence After Exposition to Cigarette Mainstream Smoke. Mycopathologia 168, 227–235 (2009). https://doi.org/10.1007/s11046-009-9217-5

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  • DOI: https://doi.org/10.1007/s11046-009-9217-5

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