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AFLP analysis and zebra disease resistance identification of 40 sisal genotypes in China

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Abstract

Sisal is the most important fiber crop in tropical and subtropical areas in China and the world. Zebra disease is a serious threat to the main cultivar Agave hybrid No.11648 (H.11648) worldwide. To select germplasm materials with zebra disease resistance for breeding, the fluorescent amplified fragment length polymorphism (AFLP) technique was used to make a cluster analysis of the genetic relationships of 40 sisal genotypes grown in China, and Phytophthora nicotianae was used to inoculate the 40 genotypes to identify their resistance to zebra disease. As a result, the similarity coefficient among 40 sisal genotypes was found to be 0.44–0.83 and the 40 genotypes show different levels of disease resistance. According to the AFLP analysis, the disease resistance and chromosomal ploidy, it can be reasoned that, A. attenuata var. marginata, Dong 109, Nan ya 1 and A. attenuata are suitable for hybridization with H.11648 to breed a new disease-resistant variety.

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Acknowledgments

This study was supported by National Nonprofit Institute Research Grant of CATAS-ITBB (No. 0827, 0828), Ministry of Agriculture Foundation 948 (No. 2010-Z6), China Agriculture Research System (CARS-19-E17) and the Natural Science Foundation of Hai Nan (No. 80510).

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Authors and Affiliations

  1. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

    Jianming Gao, Qiaolian Liu, Helong Chen, Shiqing Zhang, Chenji Jiang, Zhenzhen Dai & Kexian Yi

  2. Zhanjiang Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, 524000, China

    Luoping

  3. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, China

    Chaoming Guo, Jinlong Zheng & Kexian Yi

  4. School of Life Science, Taizhou University, Linhai, 317000, Zhejiang, China

    Jinzhi Li

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  1. Jianming Gao
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  2. Luoping
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  3. Chaoming Guo
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  4. Jinzhi Li
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  5. Qiaolian Liu
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  6. Helong Chen
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  7. Shiqing Zhang
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  8. Jinlong Zheng
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  9. Chenji Jiang
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  10. Zhenzhen Dai
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  11. Kexian Yi
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Corresponding author

Correspondence to Kexian Yi.

Additional information

Jianming Gao, Luoping, and Chaoming Guo contributed equally to the work.

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Gao, J., Luoping, Guo, C. et al. AFLP analysis and zebra disease resistance identification of 40 sisal genotypes in China. Mol Biol Rep 39, 6379–6385 (2012). https://doi.org/10.1007/s11033-012-1459-5

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  • DOI: https://doi.org/10.1007/s11033-012-1459-5

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