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Association of 1347 G/A cytochrome P450 4F2 (CYP4F2) gene variant with hypertension and stroke

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Abstract

Genetic variants of cytochrome P450 4F2 (CYP4F2) gene have been suggested to be risk factors for hypertension, cardiovascular diseases and stroke. In the present case–control study we investigated the association of 1347 G/A polymorphism (rs2108622) in the 11th exon region of CYP4F2 gene with hypertension, ischemic stroke and stroke subtypes classified according to TOAST (Trial of Org 10172 in Acute Stroke Treatment) classification. Five hundred and seven stroke patients (hypertensives: normotensives = 279:228) and four hundred and eighty seven, age and sex matched controls (males: females = 356:131) (hypertensives: normotensives = 148:339) were involved in the study. The genotypes were determined by PCR-RFLP technique. Genotypes were confirmed by subjecting the PCR products to sequencing. Significant difference was observed in the genotypic distribution and allelic frequency between the stroke patients and healthy controls. AA genotype and A allele associated significantly with stroke and hypertension [P = 0.009; OR = 1.59 (95% CI = 1.119–2.283) and P = 0.010; OR = 1.26 (95% CI = 1.056–1.502); P = 0.01; OR = 1.58 (95% CI = 1.11–2.272) and P = 0.010; OR = 1.25(95% CI = 1.054–1.504) respectively]. A stepwise logistic regression analysis confirmed these findings. To establish that this polymorphism is associated with stroke independent of hypertension; we compared stroke patients without hypertension with normotensive controls. Significant difference was observed in genotypic distribution and allelic frequency between the two groups (P = 0.001 and 0.002 respectively). Evaluating the association of this polymorphism with stroke subtypes we found significant associations with cardioembolic stroke (P < 0.001). In conclusion our study suggests that 1347A allele of CYP4F2 gene is an important risk factor for hypertension and ischemic stroke.

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Acknowledgment

Subhash kaul and Anjana Munshi are thankful to ICMR, India for financial support.

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Authors and Affiliations

  1. Department of Molecular Biology, Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500016, India

    Anjana Munshi, Vandana Sharma, Gowhar Shafi, Rajeshwar Koppula, Sai Babu Mallemoggala & A. Jyothy

  2. Department of Neurology, Nizams Institute of Medical Sciences, Punjagutta, Hyderabad, 500082, India

    Subhash Kaul

  3. Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia

    Anjana Munshi & Amal Al-Hazzani

  4. Dr. NTR University of Health Sciences, Vijayawada, Andhra Pradesh

    Vandana Sharma, Rajeshwar Koppula & Sai Babu Mallemoggala

  5. Department of Food Sciences and Nutrition, College of Food Sciences and Agriculture, King Saud University, Riyadh, Saudi Arabia

    Ali A. Alshatwi & Gowhar Shafi

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  1. Anjana Munshi
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  2. Vandana Sharma
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Correspondence to Anjana Munshi.

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Munshi, A., Sharma, V., Kaul, S. et al. Association of 1347 G/A cytochrome P450 4F2 (CYP4F2) gene variant with hypertension and stroke. Mol Biol Rep 39, 1677–1682 (2012). https://doi.org/10.1007/s11033-011-0907-y

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  • DOI: https://doi.org/10.1007/s11033-011-0907-y

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