Abstract
Higher polyphenol oxidase (PPO) activity in wheat kernels and flour has been implicated in the time dependent darkening of various end-products. Previous study conducted on a bread wheat (Triticum aestivum L.) doubled haploid (DH) mapping population derived from Chara (medium-high PPO) and WW2449 (low PPO) identified a major QTL for PPO activity located on the long arm of chromosome 2A. Physical mapping of SSR markers accounting for up to 84% of phenotypic variation for PPO activities suggests that the candidate PPO locus is localised in the deletion bin delimited by 2AL 0.77–0.85. In order to develop functional gene markers, nine wheat ESTs mapped to this deletion bin and partial PPO reference genes were explored for their sequence identities and linkage with PPO locus in a mapping population. In the present study, two markers: one SNP and one CAPS based upon BQ161439 sequence variation between the parents were identified which exhibited a tight linkage (0–0.6 cM) with the PPO loci designated as XTc1 and XPPO- LDOPA. We also mapped the reference PPO gene (GenBank AY526268) characterised from developing kernels of wheat, on the long arm of chromosome 2A which exhibited a complete linkage with XPPO- L DOPA locus. Results suggest that PPO variation displayed in the DH population from Chara/WW2449 is due to the same reference PPO gene. Allelic homoplasy of tightly linked markers, indicated that these markers are ‘diagnostic’ for the selection of low PPO gene in a range of germplasm being used in different Australian breeding programs. Identification and validation of ‘functional gene markers’ would facilitate in enhancing the selection efficiency for low PPO activity in wheat breeding programs.
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References
Anderson JV, Morris CF (2001) An improved whole-seed assay for screening wheat germplasm for polyphenol oxidase activity. Crop Sci 41:1697–1705
Bachem CWB, Speckmann GJ, van der Linde PCG, Verheggen FTM, Hunt MD, Steffens JC, Zabeau M (1994) Antisense expression of polyphenol oxidase genes inhibits enzymatic browning in potato tubers. Bio/Technology 12: 1101–1105
Baik BK, Czuchajowsa Z, Pomeranz Y (1994) Comparison of polyphenol oxidase activity in wheat and flours from Australian and US cultivars. J Cereal Sci 19:291–296
Bhattacharya M, Luo Q, Corke H (1999) Time-dependent changes in dough colour in hexaploid wheat landraces differing in polyphenol oxidase activity. J Agric Food Chem 47:3579–3585
Bowles DJ (1990) Defense-related proteins in higher plants. Annu Rev Biochem 59:873–907
Cho M-H, Moinuddin SGA, Helms GL, Hishiyama S, Eichinger D, Davin LB, Lewis NG (2003) (+)-Larreatricin hydroxylase, an enantio-specific polyphenol oxidase from creosote bush (Larrea tridentata). Proc Natl Acad Sci USA 100:10641–10646
Conley EJ, Nduati V, Gonzalez-Hernande JL, Mesfin A, Trudeau-Spanjers M et al (2004) A 2600-locus chromosome bin map of wheat homoeologous group 2 reveals interstitial gene-rich islands and colinearity with rice. Genetics 168:625–637
Constabel CP, Yip L, Patton JJ, Christopher ME (2000) Polyphenol oxidase from hybrid poplar. Cloning and expression in response to wounding and herbivory. Plant Physiol 124:285–295
Demeke T, Morris CF, Campbell KG, King GE, Anderson JA, Chang HG (2001) Wheat polyphenol oxidase: distribution and genetic mapping in three inbred line populations. Crop Sci 41:1750–1757
Felton GW, Donato K, Del vecchio RJ, Duffey SS (1989) Activation of plant foliar oxidases by insect feeding reduces nutritive quality of foliage for noctuid herbivores. J Chem Ecol 15:2667–2694
Jimenez M, Dubcovsky J (1999) Chromosome location of genes affecting polyphenol oxidase activity in seeds of common and durum wheat. Plant Breed 118:395–398
Jukanti AK, Bruckner PL, Fischer AM (2004) Evaluation of wheat polyphenol oxidase genes. Cereal Chem 81:481–485
Kosambi DD (1944) The estimation of map distances from recombination values. Annu Eugen 12:172–175
Kruger JE (1976) Changes in the polyphenol oxidases of wheat during kernel growth and maturation. Cereal Chem 53:201–213
Kruger JE, Hatcher DW, DePauw R (1994) A whole seed assay for polyphenol oxidase in Canadian prairie spring wheats and its usefulness as a measure of noodle darkening. Cereal Chem 71:324–326
Li L, Steffens JC (2002) Overexpression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance. Planta 215:239–247
Li WL, Faris JD, Chittoor JM, Leach JE, Hulbert SH, Liu DJ, Chen PD, Gill BS (1999) Genomic mapping of defence response genes in wheat. Theor Appl Genet 98:226–233
Manly KF, Cudmore Jr RH, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932
Mares DJ, Campbell AW (2001) Mapping component of flour and noodle colour in Australian wheat. Aust J Agri Res 52:1297–1309
Mayer AM, Harel E (1979) Polyphenol oxidases in plants. Phytochemistry 18:193–215
Nakayama T, Yonekura-Sakakibara K, Sato T, Kikuchi S, Fukui Y, Fukuchi-Mizutani M, Ueda T, Nakao M, Tanaka Y, Kusumi T, Nishino T (2000) Aureusidin Synthase: a polyphenol oxidase homolog responsible for flower coloration. Science 290:1163–1166
Park WJ, Shelton DR, Peterson CJ, Martin TJ, Kachman SD, Wehling RL (1997) Variation in polyphenol oxidase activity and quality characteristics among hard white wheat and hard red winter wheat samples. Cereal Chem 74:7–11
Raman R, Raman H, Johnstone K, Lisle C, Smith A, Martin P, Allen H (2005) Genetic and in silico comparative mapping of the polyphenol oxidase gene in bread wheat. Funct Integ Genomics 5:185–200
Rani KU, Rao UJSP, Leelavathi K, Rao PH (2001) Distribution of enzymes in wheat flour mill streams. J Cereal Sci 34:233–242
Robb DA (1984) Copper protein and copper enzyme, vol 2. In: Lontie R (ed) CRC Press. Boca Raton, FL, pp 207–241
Röder MS, Korzun V, Gill BS, Ganal MW (1998a) The physical mapping of microsatellite markers in wheat. Genome 41:278–283
Röder MS, Korzum V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW (1998b) A microsatellite map of wheat. Genetics 149:2007–2023
Sears ER (1966) Nullisomic-tetrasomic combinations in hexaploid wheat. In: Riley R, Lewis KR (eds) Chromosome manipulation and plant genetics. Oliver and Boyd, Edinburgh, pp 29–45
Sheptovitsky YG, Brudvig GW (1996) Isolation and characterisation of spinach photosystem II membrane-associated catalase and polyphenol oxidase. Biochemistry 35:16255–16263
Simeone R, Pasqualone A, Clodoveo ML, Blanco A (2002) Genetic mapping of polyphenol oxidase in tetraploid wheat. Cell Mol Biol Lett 7:763–769
Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114
Sorrells ME, Rota ML, Bermudez-Kandianis CE, Greene RA, Kantety R, Munkvold JD, Miftahudin, Mahmoud A, Ma X, Gustafson PJ, Qi LL, Echalier B, Gill BS, Matthews DE, Lazo GR, Chao S, Anderson OD, Edwards H, Linkiewicz AM, Dubcovsky J, Akhunov ED, Dvorak J, Zhang D, Nguyen HT, Peng J, Lapitan NLV, Gonzalez-Hernandez JL, Anderson JA, Hossain K, Kalavacharla V, Kianian SF, Choi D-W, Close TJ, Dilbirligi M, Gill KS, Steber C, Walker-Simmons MK, McGuire PE, Qualset CO (2003) Comparative DNA sequence analysis of wheat and rice genomes. Genome Res 13:1818–1827
Sourdille P, Singh S, Cadalen T, Brown-Guedira Gina L, Gay G, Qi L, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationship in wheat Triticum aestivum L. Funct Integr Genomics 4:12–25
Souza E, Windes J, Udall J (1998) Inheritance of polyphenol oxidase in three recombinant inbred populations of wheat. In: 1998 annual meeting abstracts. ASA, CSSA, and SSSA, Madison, WI, 67 pp
Sullivan ML, Hatfield RD, Thoma SL, Samac DA (2004) Cloning and characterisation of red clover polyphenol oxidase cDNA and expression of active protein in Escherichia coli and transgenic alfalfa. Plant Physiol 136:3234–3244
Sun DJ, He ZH, Xia XC, Zhang LP, Morris CF, Appels R, Ma WJ, Wang H (2005) A novel marker for polyphenol oxidase activity in bread wheat. Mol Breeding 16:209–218
Taneja SR, Abrol YP, Sachar RC (1974) Modulation of o-diphenolase and monophenolase enzymes during wheat grain development. Cereal Chem 51:457–465
Thipyapong P, Steffens JC (1997) Tomato polyphenol oxidase: Differential response of the polyphenol oxidase F promoter to injuries and wound signals. Plant Physiol 115:409–418
Udall J (1997) Important alleles for noodle quality in winter wheat as identified by molecular markers. MS Thesis, University of Idaho, Moscow, ID
Voorrips RE (2002) MapChart: Software for the graphical presentation of linkage maps and QTLs. J Heredity 93(1):77–78
Watanabe N, Takeuchi A, Nakayama A (2004) Inheritance and chromosomal location of homoeologous genes affecting phenol colour reaction of kernels in durum wheat. Euphytica 139:87–93
Weber JL (1990) Informativeness of human (dC-dA)n (dG-dT)n polymorphisms. Genomics 7:524–530
Wrigley CW, McIntosh RA (1975) Genetic control of factors regulating the phenol reaction of wheat and rye grain. Wheat Inform Serv 40:6–11
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Support from the NSW Agricultural Genomics Centre under BioFirst Initiative of NSW Government, Australia is gratefully acknowledged.
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Raman, R., Raman, H. & Martin, P. Functional gene markers for polyphenol oxidase locus in bread wheat (Triticum aestivum L.). Mol Breeding 19, 315–328 (2007). https://doi.org/10.1007/s11032-006-9064-8
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DOI: https://doi.org/10.1007/s11032-006-9064-8