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T3 and T4 decrease ROS levels and increase endothelial nitric oxide synthase expression in the myocardium of infarcted rats

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Abstract

Myocardial infarction leads to a reduction in nitric oxide (NO) bioavailability and an increase in reactive oxygen species (ROS) levels. This scenario has been shown to be detrimental to the heart. Recent studies have shown that thyroid hormone (TH) administration presents positive effects after ischaemic injury. Based on this, the aim of this study was to evaluate the effect of TH on NO bioavailability as well as on endothelial nitric oxide synthase (eNOS) expression after myocardial infarction. Male Wistar rats were divided into three groups: Sham-operated (SHAM), infarcted (AMI) and infarcted + TH (AMIT). During 26 days, the AMIT group received T3 and T4 (2 and 8 µg/100 g/day, respectively) by gavage, while SHAM and AMI rats received saline. After this, the rats underwent echocardiographic analysis were sacrificed, and the left ventricle was collected for biochemical and molecular analysis. Statistical analysis: one-way ANOVA with Student–Newman–Keuls post test. AMI rats presented a 38 % increase in ROS levels. TH administration prevented these alterations in AMIT rats. The AMIT group presented an increase in eNOS expression, in NOS activity and in nitrite levels. TH administration also increased PGC-1α expression in the AMIT group. In conclusion, TH effects seem to involve a modulation of eNOS expression and an improvement in NO bioavailability in the infarcted heart.

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Acknowledgments

This work was supported by the Brazilian Research Agencies: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenacão de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação de Amparo à Pesquisa do Rio Grande do Sul (FAPERGS). We are indebted to Dr. Constantinos Pantos and to Dr. Iordanis Mourouzis for their full assistance.

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Authors and Affiliations

  1. Laboratório de Fisiologia Cardiovascular, Departamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite 500, sala 01, Porto Alegre, RS, CEP 90050170, Brazil

    Alexandre Luz de Castro, Angela Vicente Tavares, Rafael Oliveira Fernandes, Cristina Campos, Adriana Conzatti, Rafaela Siqueira, Tânia Regina G. Fernandes, Carmem L. Sartório, Adriane Belló-Klein & Alex Sander da Rosa Araujo

  2. Departamento de Fisiologia e Farmacologia, Universidade Federal de Pelotas, Campus Universitário Capão do Leão, Pelotas, RS, CEP 96900010, Brazil

    Paulo Cavalheiro Schenkel

  3. Universidad de Buenos Aires, C1053ABJ, Buenos Aires, Argentina

    Susana Llesuy

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  1. Alexandre Luz de Castro
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  2. Angela Vicente Tavares
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Correspondence to Alex Sander da Rosa Araujo.

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The authors declare that there are no conflicts of interest. The authors declare that the experimental procedures were performed taking into consideration the welfare of animals and were approved by the Ethics Committee for animal research at Universidade Federal do Rio Grande do Sul.

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de Castro, A.L., Tavares, A.V., Fernandes, R.O. et al. T3 and T4 decrease ROS levels and increase endothelial nitric oxide synthase expression in the myocardium of infarcted rats. Mol Cell Biochem 408, 235–243 (2015). https://doi.org/10.1007/s11010-015-2501-4

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  • DOI: https://doi.org/10.1007/s11010-015-2501-4

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