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Pyranocoumarins Isolated from Peucedanum praeruptorum Dunn Suppress Lipopolysaccharide-Induced Inflammatory Response in Murine Macrophages Through Inhibition of NF-κB and STAT3 Activation

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Abstract

Praeruptorin C, D, and E (PC, PD, and PE) are three pyranocoumarins isolated from the dried root of Peucedanum praeruptorum Dunn of Umbelliferae. In the present study, we investigated the anti-inflammatory effect of these compounds in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Pyranocoumarins significantly inhibited LPS-induced production of nitric oxide, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α). The mRNA and protein expressions of inducible nitric oxide synthase, IL-6, and TNF-α were also suppressed by these compounds. Both PD and PE exhibited greater anti-inflammatory activities than PC. Further study showed that pyranocoumarins suppressed the cytoplasmic loss of inhibitor κB-α protein and inhibited the translocation of NF-κB from cytoplasm to nucleus. In addition, pyranocoumarins suppressed LPS-induced STAT3 tyrosine phosphorylation. Taken together, the results suggest that pyranocoumarins may exert anti-inflammatory effects in LPS-stimulated RAW 264.7 macrophages through the inhibition of NF-κB and STAT3 activation.

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Acknowledgment

This work was supported by grants from the Science and Technology Bureau of Guangzhou (2006Z1-E6021), the National Natural Science Foundation of China (no. 30901989), and the Natural Science Foundation of Guangdong Province (no. 9151063201000008)

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  1. School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China

    Peng-Jiu Yu, Hong Jin, Jun-Yan Zhang, Guang-Fa Wang, Jing-Rong Li, Zheng-Guang Zhu, Yuan-Xin Tian, Shao-Yu Wu, Wei Xu, Jia-Jie Zhang & Shu-Guang Wu

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Correspondence to Jia-Jie Zhang or Shu-Guang Wu.

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Peng-Jiu Yu and Hong Jin contributed equally to this paper.

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Yu, PJ., Jin, H., Zhang, JY. et al. Pyranocoumarins Isolated from Peucedanum praeruptorum Dunn Suppress Lipopolysaccharide-Induced Inflammatory Response in Murine Macrophages Through Inhibition of NF-κB and STAT3 Activation. Inflammation 35, 967–977 (2012). https://doi.org/10.1007/s10753-011-9400-y

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