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Association of reduced XRCC2 expression with lymph node metastasis in breast cancer tissues

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Abstract

The main purpose of this study was to evaluate the association between reduction in XRCC2 gene and involvement of lymph node metastasis in breast cancer. In first part of the study, meta-analysis of 14 published XRCC2 studies was performed to define the role of XRCC2 gene as diagnostic marker and in second part of the study XRCC2 gene expression was observed using real time PCR in study cohort of 100 females (50 breast cancer patients and 50 controls). A statistically significant down regulation of XRCC2 (p < 0.04) and up-regulation of ki-67 (p < 0.05) was observed in breast cancer tissues compared to non-cancerous healthy tissues. In order to explore gene–gene and gene-clinicopathological parameters relationship Spearmen correlation was performed. We observed a significantly negative correlation between XRCC2 and Ki-67 expression (r = −0.376**, p < 0.01). In case of gene-clinicopathological parameters relationship, we observed a significant correlation between XRCC2 expression and lymph node status (r = −0.521***, p < 0.002) and metastatic status (r = −0.303*, p < 0.04) of breast cancer patients. Our data suggests that deregulation of XRCC2 in breast cancer has the potential to predict lymph node metastasis and may serve as a therapeutic target for breast cancer patients at risk of metastasis.

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Acknowledgments

All authors wish to acknowledge the patients and control individuals who contributed to this research work. We are also thankful to hospital staff (Nuclear Oncology and Radiotherapy Institute Islamabad (NORI) and Military Hospital Rawalpindi (MH) Pakistan, for their help and cooperation.

Conflict of interest

Authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Cancer Genetics Lab, Department of Biosciences, COMSATS Institute of Information Technology, Park Road, Chakshazad, Islamabad, Pakistan

    Nabiha Bashir, Syeda Sana, Ishrat Mahjabeen & Mahmood Akhtar Kayani

Authors
  1. Nabiha Bashir
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  2. Syeda Sana
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  3. Ishrat Mahjabeen
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  4. Mahmood Akhtar Kayani
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Corresponding author

Correspondence to Mahmood Akhtar Kayani.

Additional information

Nabiha Bashir and Syeda Sana have contributed equally to this work.

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Bashir, N., Sana, S., Mahjabeen, I. et al. Association of reduced XRCC2 expression with lymph node metastasis in breast cancer tissues. Familial Cancer 13, 611–617 (2014). https://doi.org/10.1007/s10689-014-9745-0

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