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A differential response to chemical elicitors in Catharanthus roseus in vitro cultures

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Abstract

The effects of methyl jasmonate, salicylic acid and ethylene on alkaloid accumulation in in vitro cell suspension, hairy roots and rootless shoot cultures of Catharanthus roseus were analyzed. Ajmalicine, but not catharanthine, accumulation was promoted by jasmonate and ethylene treatments in cell suspensions. In hairy roots, jasmonate induced the accumulation of both alkaloids, whereas ethylene only induced catharanthine accumulation. In shoot cultures, positive effects of jasmonate and ethylene were recorded only in vindoline accumulation. Ethylene diminished catharanthine accumulation in these cultures. No effect of salicylic acid was observed in any of the studied in vitro culture systems.

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Abbreviations

ET:

Ethylene

MeJa:

Methyl jasmonate

SA:

Salicylic acid

TDC:

Tryptophan decarboxylase

References

  • De Luca V, St-Pierre B (2000) The cell and developmental biology of alkaloid biosynthesis. Trends Plant Sci 5:349–364

    Article  Google Scholar 

  • Hernández-Domínguez E, Vázquez-Flota F (2006) Monoterpenoid alkaloid quantitation by in situ densitometry-thin layer chromatography. J Liq Chromatogr Relat Technol 29:583–590

    Article  Google Scholar 

  • Hernández-Domínguez E, Campos-Tamayo F, Vázquez-Flota F (2004) Vindoline synthesis in in vitro shoot cultures of Catharanthus roseus. Biotechnol Lett 26:671–674

    Article  PubMed  Google Scholar 

  • Islas-Flores I, Loyola-Vargas VM, Miranda-Ham ML (1994) Tryptophan decarboxylase in transformed roots from Catharanthus roseus and its relationship to tryptamine ajmalicine, and catharanthine accumulation during the culture cycle. In vitro Cell Dev Biol 30P:81–83

    Google Scholar 

  • Rijhwani SK, Shanks JV (1998) Effects of elicitor dosage and exposure time on biosynthesis of indole alkaloids by Catharanthus roseus hairy root cultures. Biotechnol Prog 14:442–449

    Article  PubMed  CAS  Google Scholar 

  • van der Heijden L, Jacobs DI, Snoeijer W, Hallard D, Verpoorte R (2004) The Catharanthus alkaloids: pharmacology and biotechnology. Curr Med Chem 11:607–628

    Article  Google Scholar 

  • Vázquez-Flota F, Loyola-Vargas VM (2003) In vitro plant cell culture as the basis for the development of a research institute in Mexico: Centro de Investigación Científica de Yucatán. In vitro Cell DevBiol 39P:250–258

    Article  Google Scholar 

  • Vázquez-Flota F, Moreno-Valenzuela O, Miranda-Ham ML, Coello-Coello J, Loyola-Vargas VM (1994) Catharanthine and ajmalicine synthesis in Catharanthus roseus hairy roots cultures. Medium optimization and elicitation. Plant Cell Organ Cult 38:273–279

    Article  Google Scholar 

  • Vázquez-Flota F, Carrillo-Pech M, Minero-García Y, Miranda-Ham ML (2004) Alkaloid metabolism in wounded Catharanthus seedlings. Plant Physiol Biochem 42:623–628

    Article  PubMed  Google Scholar 

  • Whitmer S, Canel C, Hallard D, Goncalves C, Verpoorte R (1998) Influence of precursor availability on alkaloid accumulation by transgenic cell line of Catharanthus roseus. Plant Physiol 116:853–857

    Article  PubMed  CAS  Google Scholar 

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Acknowledgment

Supported by the National Council of Science and Technology (CONCAYT, México).

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Authors and Affiliations

  1. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 No. 130 Col. Chuburná, Merida, Yucatan, 97200, Mexico

    Felipe Vázquez-Flota, Elizabeta Hernández-Domínguez, Ma. de Lourdes Miranda-Ham & Miriam Monforte-González

Authors
  1. Felipe Vázquez-Flota
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  2. Elizabeta Hernández-Domínguez
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  3. Ma. de Lourdes Miranda-Ham
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  4. Miriam Monforte-González
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Corresponding author

Correspondence to Felipe Vázquez-Flota.

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10529_2008_9881_MOESM1_ESM.doc

Alkaloid accumulation in C. roseus cell suspension (A), hairy roots (B) and rootless shoots (C) cultures exposed to salicylic acid (1 mM) for the indicated times. Values above the bars corresponding to control indicate the alkaloid content taken as 100%. Average of triplicates, standard deviation was within 9 % of the mean. Seven day-old cell, ten-day old root, and twelve-day old shoot cultures were employed. (DOC 58 kb)

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Vázquez-Flota, F., Hernández-Domínguez, E., de Lourdes Miranda-Ham, M. et al. A differential response to chemical elicitors in Catharanthus roseus in vitro cultures. Biotechnol Lett 31, 591–595 (2009). https://doi.org/10.1007/s10529-008-9881-4

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  • DOI: https://doi.org/10.1007/s10529-008-9881-4

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