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Apoptosis, necrosis and autophagy are influenced by metabolic energy sources in cultured rat spermatocytes

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Abstract

Apoptosis, necrosis and autophagy are mechanistically related processes that control tissue homeostasis and cell survival. In the testis, germ cell death is important for controlling sperm output, but it is unknown whether or not germ cells can switch from apoptosis to necrosis, as has been reported in other tissues. Furthermore, autophagy has not been reported in spermatogenesis. Spermatocytes (meiotic cells) and spermatids (haploid cells) use lactate rather than glucose as their primary substrate for producing ATP. The metabolism of glucose, but not lactate, reduces ATP levels and increases intracellular [H+] and [Ca2+], both of which are associated with apoptosis and/or necrosis in somatic cells. In this work, we evaluated whether different energy sources, such as lactate or glucose, can influence spermatocyte death type and/or survival in primary cultures. Spermatocytes cultured for 12 h without an energy source died by necrosis, while spermatocytes cultured with 5 mM glucose showed a significant increase in apoptosis, as evidenced by caspase activity, TUNEL assay and phosphatidylserine exposure. Apoptosis was not observed in spermatocytes cultured with 5 mM lactate or deoxyglucose. Authophagy markers, such as LC3-II and autophagosomes, were detected after 12 h of culture, regardless the culture conditions. These results suggest that the availability of glucose and/or lactate affect the type of death or the survival of primary spermatocytes, where glucose can induce apoptosis, while lactate is a protective factor.

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Acknowledgments

Part of this work was supported by a grant from the Chilean Research Council (FONDECYT, 1110778 and 1110267) to RDM and JGR and FONDAP 15010006 to SL.

Conflict of interest

None declared.

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Author notes

  1. Ximena Bustamante-Marín

    Present address: Departamento Biomédico, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Antofagasta, Chile

Authors and Affiliations

  1. Departamento de Fisiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, Santiago, Chile

    Ximena Bustamante-Marín & Ricardo D. Moreno

  2. Centro Estudios Moleculares de la Celula, Facultad Ciencias Quimicas y Farmaceuticas/Facultad Medicina, Universidad de Chile, Santiago, Chile

    Clara Quiroga & Sergio Lavandero

  3. Department of Internal Medicine (Cardiology Division), University of Texas Southwestern Medical Center, Dallas, Texas, USA

    Clara Quiroga & Sergio Lavandero

  4. Instituto de Química, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile

    Juan G. Reyes

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  1. Ximena Bustamante-Marín
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Corresponding author

Correspondence to Ricardo D. Moreno.

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Bustamante-Marín, X., Quiroga, C., Lavandero, S. et al. Apoptosis, necrosis and autophagy are influenced by metabolic energy sources in cultured rat spermatocytes. Apoptosis 17, 539–550 (2012). https://doi.org/10.1007/s10495-012-0709-2

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