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Metabolomic Analysis of the Lichen Everniopsis trulla Using Ultra High Performance Liquid Chromatography-Quadrupole-Orbitrap Mass Spectrometry (UHPLC-Q-OT-MS)

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Abstract

A new depside was identified in the methanolic extract from the lichen Everniopsis trulla based on the metabolomics UHPLC–DAD–MS analysis and HESI–MS–MS fragmentation patterns along with thirty-two known compounds for the first time. The compounds were structurally characterized by UV and high resolution quadrupole orbitrap mass spectra and by comparison with literature. According to the characteristic fragmentation patterns, the presence of two simple aromatic compounds, six lipid derivatives, eight depsidones, thirteen depsides, a chromone, two diphenylethers, and a dibenzofuran were identified. To our knowledge, this is the first study of the lichen E. trulla by liquid chromatography hyphenated with tandem mass spectrometry.

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Acknowledgements

The work was supported by FONDECYT REGULAR 1150745. We are grateful to Prof. Magda Chanco for the identification of the lichen under study. Mario Simirgiotis acknowledge FONDEQUIP EQM 140002.

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Authors and Affiliations

  1. Departamento de Química, Faculty of Sciences and Engineering, Pontifical Catholic University of Peru, Av. Universitaria 1801, Lima 32, Lima, Peru

    Olivio N. Castro

  2. Facultad de Ciencias de la Salud, Universidad Arturo Prat, Iquique, Chile

    Julio Benites

  3. Departamento de Química, Universidad da Beira Interior, Covilhã, Portugal

    Jesus Rodilla

  4. Departamento de Química, Universidad Nacional Mayor de San Marcos, Lima, Peru

    Julio C. Santiago

  5. Instituto de Farmacia, Facultad de Ciencias, Universidad Austral de Chile, Casilla 567, Valdivia, 5090000, Chile

    Mario Simirgiotis

  6. Departamento de Ciencias Químicas, Universidad Andrés Bello, Campus Viña del Mar, Quillota 980, Viña del Mar, 2520000, Chile

    Beatriz Sepulveda

  7. Departamento de Química, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile

    Carlos Areche

Authors
  1. Olivio N. Castro
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  2. Julio Benites
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  3. Jesus Rodilla
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  4. Julio C. Santiago
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  5. Mario Simirgiotis
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  6. Beatriz Sepulveda
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  7. Carlos Areche
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Corresponding authors

Correspondence to Olivio N. Castro or Carlos Areche.

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Castro, O.N., Benites, J., Rodilla, J. et al. Metabolomic Analysis of the Lichen Everniopsis trulla Using Ultra High Performance Liquid Chromatography-Quadrupole-Orbitrap Mass Spectrometry (UHPLC-Q-OT-MS). Chromatographia 80, 967–973 (2017). https://doi.org/10.1007/s10337-017-3304-4

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  • DOI: https://doi.org/10.1007/s10337-017-3304-4

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