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Direct pulp capping effect with experimentally developed adhesive resin systems containing reparative dentin-promoting agents on rat pulp: mixed amounts of additives and their effect on wound healing

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Abstract

This study examined the wound-healing process of exposed rat pulp when treated with experimental adhesive resin systems. The experimental direct pulp capping adhesive resin systems were composed of primer-I, primer-II and an experimental bonding agent. Primer-I was Clearfil SE Bond (CSE) primer containing 1.0 or 5.0 wt% CaCl2, and primer-II was CSE primer containing 0.1, 1.0 or 5.0 wt% compound of equal mole of pA and pB with synthetic peptides derived from dentin matrix protein 1 (DMP1). Primer-I containing 1.0 and 5.0 wt% CaCl2 was assigned to the experimental groups 1–3 and 4–6, respectively. Primer-II containing 0.1, 1.0 or 5.0 wt% compound of pA and pB was assigned to the experimental groups 1 and 4, 2 and 5, and 3 and 6, respectively. In all experimental groups, CSE bond containing 10 wt% hydroxyapatite powder was used as the experimental bonding agent. The positive control teeth were capped with calcium hydroxide preparation (Dycal), and the negative control teeth were capped with CSE. The specimens were alternately stained with Mayer’s H&E and the enhanced polymer one-step staining methods. Experimental groups 1, 4, 5 and 6 showed a higher level of reparative dentin formation compared to the negative control 14 days postoperatively. At 28 days postoperatively, all experimental groups showed the formation of extensive reparative dentin, and experimental groups 4, 5 and 6 demonstrated similar dentin bridge formation as that of the positive control. How quickly reparative dentin formation occurs may depend on the concentration of CaCl2 and the compound of pA and pB in the experimental primer.

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Acknowledgments

The authors thank Kuraray Dental Inc. for the experimental adhesive resins systems and other materials they generously provided.

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Authors and Affiliations

  1. Advanced Operative Dentistry-Endodontics, The Nippon Dental University Graduate School of Life Dentistry at Niigata, Niigata, Japan

    Yoshihisa Taira

  2. Department of Operative Dentistry, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan

    Yoshihisa Taira, Koichi Shinkai, Masaya Suzuki, Chikage Kato & Yoshiroh Katoh

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  1. Yoshihisa Taira
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Correspondence to Yoshihisa Taira.

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Taira, Y., Shinkai, K., Suzuki, M. et al. Direct pulp capping effect with experimentally developed adhesive resin systems containing reparative dentin-promoting agents on rat pulp: mixed amounts of additives and their effect on wound healing. Odontology 99, 135–147 (2011). https://doi.org/10.1007/s10266-011-0006-5

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