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Laser irradiation affects enzymatic antioxidant system of streptozotocin-induced diabetic rats

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Abstract

The aim of the present study was to analyze the effect of low-power laser irradiation in the antioxidant enzymatic system of submandibular (SMG) and parotid (PG) salivary glands of streptozotocin-induced diabetic rats. The animals were randomly divided into six groups: three diabetic groups (D0, D5, and D20) and three non-diabetic groups (C0, C5, and C20), according to laser dose received (0, 5, and 20 J/cm2, respectively). Areas of approximately 1 cm2 were demarcated in the salivary glands (each parotid and both submandibular glands) and after irradiated according to Simões et.al. (Lasers Med Sci 24:202–208, 2009). A diode laser (660 nm/100 mW) was used, with laser beam spot of 0.0177 cm2. The group treated with 5 J/cm2 laser dose was subjected to irradiation for 1 min and 4 s (total irradiation time) and the group treated with 20 J/cm2 laser dose was subjected to irradiation for 4 min and 16 s. Twenty-four hours after irradiation the animals were euthanized and the salivary glands were removed for biochemical analysis. The total antioxidant values (TA), the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase enzymes were determined. SOD and CAT activities, as well as TA were higher in SMG of irradiated diabetic rats. However, in SMG of non-diabetic rats, laser irradiation decreased TA values and led to an increase in the CAT activity. In addition, there was a decrease in the activity of CAT in PG of diabetic and non-diabetic animals after laser irradiation. According to the results of the present study, low-power laser irradiation can affect the enzymatic antioxidant system of salivary glands of streptozotocin-induced diabetic rats.

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Acknowledgments

The authors wish to express their gratitude to the Special Laboratory of Lasers in Dentistry (LELO) and to FAPESP (Fundação de Apoio a Pesquisa do Estado de São Paulo, #08/009787-5 and #2011/14013-1), for financial support.

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Authors and Affiliations

  1. Departamento de Biomateriais e Biologia Oral, Faculdade de Odontologia, Universidade de São Paulo (USP), São Paulo, Brazil, 05508-000

    Flavia Kazue Ibuki, Alyne Simões, José Nicolau & Fernando Neves Nogueira

  2. Laboratório de Biologia Oral, Faculdade de Odontologia, Cidade Universitária, Av. Prof. Lineu Prestes, 2227, São Paulo, SP, Brazil, 05508-900

    Alyne Simões

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  1. Flavia Kazue Ibuki
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  2. Alyne Simões
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  3. José Nicolau
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  4. Fernando Neves Nogueira
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Correspondence to Alyne Simões.

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Ibuki, F.K., Simões, A., Nicolau, J. et al. Laser irradiation affects enzymatic antioxidant system of streptozotocin-induced diabetic rats. Lasers Med Sci 28, 911–918 (2013). https://doi.org/10.1007/s10103-012-1173-5

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  • DOI: https://doi.org/10.1007/s10103-012-1173-5

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